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-  2018 

槲皮苷对IL-1β诱导的软骨细胞凋亡的影响及其机制 Effect of Quercetin on Chondrocyte Apoptosis Induced by Interleukin 1 Beta and Its Mechanism

Keywords: 槲皮苷,白细胞介素-1β,软骨细胞,凋亡

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Abstract:

目的:探讨槲皮苷对白细胞介素-1β(IL-1β)诱导的软骨细胞凋亡的影响及其可能作用机制。方法:选取8周龄SD大鼠5只提取软骨细胞,经鉴定后,将软骨细胞随机分为5组,每组5孔,重复3次。对照组(不施加任何干预),IL-1β组(50μg/ml IL-1β作用于软骨细胞24h),槲皮苷组(IL-1β组基础上同时施加100,200,400μmol/L槲皮苷)。观察5组软骨细胞凋亡率,同时应用聚合酶链式反应(PCR)以及免疫印记法(Western Blot)检测凋亡相关基因与蛋白Bcl-2、Bax以及Caspase 9的表达量。酶联免疫法(ELISA)检测各组诱导型一氧化氮合酶(iNOS)的表达情况。结果:与对照组比较,IL-1β组、100,200,400μmol/L槲皮苷组处理后软骨细胞的凋亡率以及iNOS表达量明显增加,Bax以及Caspase 9的基因表达量显著升高,而抗凋亡基因Bcl-2表达量明显下降,差异均有统计学意义(P<0.05);与IL-1β组比较,400μmol/L槲皮苷组处理后可以显著降低软骨细胞凋亡率以及iNOS表达量,Bax以及Caspase 9的基因以及蛋白表达量明显下降,而抗凋亡基因与蛋白Bcl-2表达量明显升高,差异有统计学意义(P<0.05)。结论:槲皮苷可以通过减少Bax的表达以及增加Bcl-2的表达进而减少Caspase 9的表达,从而抑制IL-1β导致的软骨细胞凋亡

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