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-  2018 

缺氧诱导因子-2α对滋养细胞HTR8/SVneo侵袭及血管形成的影响 Effect of Hypoxia-Inducible Factor 2 Alpha on the Invasion and Tube Formation of Extravillour Trophoblast Cell Line HTR8/SVneo

Keywords: 缺氧,子痫前期,侵袭,血管形成,HIF-2α,ARRDC3,PPARγ

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Abstract:

目的:研究缺氧诱导因子2α(HIF-2α)对绒膜外滋养细胞系HTR8/SVneo侵袭及血管形成的影响,并探讨其可能的信号通路。方法:建立滋养细胞缺氧模型,用Western Blot检测HIF-2α蛋白的表达、Transwell小室法检测侵袭能力、Tube formation法检测血管形成能力。同时应用HIF-2α抑制剂检测缺氧条件下HIF-2α表达抑制对绒毛膜外滋养细胞HTR8/SVneo侵袭及血管形成的影响,并检测其下游信号因子ARRDC3及PPARγ的表达。结果:缺氧(1%O2)诱导HTR8/SVneo细胞HIF-2α的表达并抑制其侵袭及血管形成能力;缺氧条件下(1%O2),HIF-2α抑制剂抑制HTR8/SVneo细胞HIF-2α的表达,改善缺氧对HTR8/SVneo细胞侵袭及管形成的抑制,并下调ARRDC3及PPARγ的表达。结论:HIF-2α可能通过介导ARRDC3和PPARγ的表达抑制滋养细胞侵袭及血管形成,为子痫前期发病机制的研究提供了新线索

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