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-  2017 

大鼠颅脑损伤中肿瘤坏死因子-α及核转录因子-κB的变化及其意义 TNF-Alpha and NF-Kappa B Changes in Brain Tissue and Their Significance in Secondary Brain Injury of Rats

Keywords: TNF-α,NF-κB,颅脑损伤

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Abstract:

目的:通过观察肿瘤坏死因子(TNF-α)以及核转录因子κB(NF-κB)在颅脑损伤后脑组织中的表达变化,初步探讨两者在颅脑损伤后继发性脑损伤中的意义。方法:健康成年雄性SD大鼠64只,体重230-310g,随机分成对照组8只和颅脑损伤组56只,颅脑损伤组在外伤后1h、3h、6h、12h、24h、3d、7d处死,每组处死8只。取损伤灶周围的脑组织进行含水量以及超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽(GSH)的测定,并进行病理切片观察其病理变化,最后用免疫组化法检测脑组织中TNF-α和NF-κB的含量。结果:1成功构建大鼠颅脑损伤的模型,颅脑损伤后的各时间点神经功能评分差异无统计学意义(P>0.05);2颅脑损伤组大鼠的脑组织含水量较对照组显著升高,并且在3d达到高峰,7d时逐渐下降,但仍高于对照组,差异有统计学意义(P<0.05);3大鼠颅脑损伤后切片表现为有大量的炎症细胞积聚,并有不同程度的脑细胞水肿,随着时间的推移逐渐加重,在第7d时,水肿较之前减轻,但仍有大量的炎性细胞浸润以及少量的纤维组织增生;4大鼠颅脑损伤后1h开始,损伤灶周围的脑组织中MDA的含量显著升高,且在之后的时间内持续升高,在3d时达到高峰,随后下降,7d时表达仍然高于对照组,而损伤组的SOD活性及GSH的活力明显降低,在7d时仍低于对照组,差异有统计学意义(P<0.05);5TNF-α以及NF-κB在颅脑损伤组中含量显著高于对照组,且在颅脑损伤后1h开始升高,在3d左右达到高峰,7d时稍有降低,但仍高于对照组,为高表达状态。结论:颅脑损伤后TNF-α以及NF-κB的变化趋势与继发性脑损伤的临床表现一致,参与了继发性脑损伤的病理过程

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