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-  2018 

家兔脑死亡状态通过蛋白激酶C途径介导小肠细胞凋亡 Brain Death Mediates the Apoptosis of Small Intestinal Cells Through PKC Pathway

Keywords: 脑死亡,小肠移植,蛋白激酶C,Caspase3,Bax

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Abstract:

目的:通过建立脑死亡模型,探讨小肠细胞蛋白激酶C(PKC)与凋亡相关基因表达的关系。方法:60只家兔随机均分为两组,即假手术组和脑死亡组。两组均在脑死亡后2,6,8h采集小肠标本,苏木精-伊红染色观察小肠结构改变,免疫组织化学法检测Caspase3,PKC和NF-κB的蛋白表达,RT-PCR检测Bax mRNA表达。结果:假手术组小肠损伤不明显,脑死亡组随着时间的延长,小肠损伤逐渐加重。Caspase3,PKC,NF-κB蛋白表达量,Bax mRNA表达量均随着脑死亡时间延长而增高(0.30±0.04,0.63±0.04,1.32±0.07,P<0.05),且各时间点脑死亡组四个指标表达量均高于假手术组。结论:脑死亡状态影响小肠组织结构改变,且随着脑死亡时间的延长,PKC及凋亡相关基因的表达量均增高。脑死亡可能通过PKC途径介导小肠细胞凋亡过程

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