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-  2017 

柚皮素减轻AngⅡ诱导的原代大鼠心肌细胞肥大作用 Naringenin Alleviates Angiotensin Ⅱ Induced Neonatal Rat Ventricular Cardiomyocytes Hypertrophy

Keywords: 柚皮素,心肌肥大,MAPK,细胞凋亡

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Abstract:

目的:探讨柚皮素(Naringenin)对血管紧张素Ⅱ(AngⅡ)诱导的原代大鼠心肌细胞(NRVMs)肥大作用及其作用机制。方法:AngⅡ刺激NRVMs构建体外心肌肥大模型,分为Vehicle组、Naringenin组、AngⅡ组和AngⅡ+Naringenin组。CCK8检测心肌细胞活性,α-actinin免疫荧光染色检测心肌细胞横截面积,RT-PCR检测ANP、BNP mRNA表达水平,Western Blot检测JNK、ERK及P38蛋白磷酸化水平,Hoechst染色检测心肌细胞凋亡。结果:与对照组相比,AngⅡ组心肌细胞横截面积明显增大,ANP、BNP mRNA表达水平明显增加,给予柚皮素干预后心肌细胞面积减小,ANP、BNP mRNA表达水平降低;此外,柚皮素能够减轻AngⅡ诱导的ERK和P38蛋白磷酸化水平上调及心肌细胞凋亡。结论:柚皮素可以减轻AngⅡ刺激引起的心肌细胞肥大,其机制可能与抑制MAPK信号通路以及减轻心肌细胞凋亡有关

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