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-  2016 

脂肪间充质干细胞培养上清对脑胶质瘤细胞凋亡、侵袭的影响 Effect of Conditional Supernatant of Human Adipose Derived Mesenchymal Stem Cells on the Apoptosis and Invasion of Human Glioma Cell Line

Keywords: 脂肪间充质干细胞,胶质瘤细胞,凋亡,侵袭

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Abstract:

目的:了解脂肪间充质干细胞(ASC)的培养上清的抗胶质瘤特性。方法:使用ASC培养上清培养胶质瘤细胞U251、U87,采用CCK-8检测ASC的培养上清抑制胶质瘤细胞U251、U87的增殖能力。利用流式细胞仪检测Annexin-Ⅴ的表达分析U251、U87在ASC上清诱导培养后的凋亡程度。划痕试验、Matrigel侵袭试验检测ASC的培养上清降低U251、U87侵袭的能力。结果:脂肪间充质干细胞能抑制胶质瘤U251、U87细胞的增殖,Annxin-V和PI的凋亡检测表明ASC-CM能诱导U251、U87细胞凋亡。划痕实验和Metrigel实验显示U251、U87细胞的迁移性降低和侵袭能力减弱。结论:脂肪间充质干细胞培养上清均能有效诱导胶质瘤U251、U87细胞的凋亡,并且均能降低胶质瘤细胞迁移及侵袭能力。我们的发现提示ASC培养上清有良好的抗肿瘤的特性,并可能用于未来的胶质瘤的治疗

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