全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...
-  2015 

IQGAP1在调节足细胞细胞骨架重组中的作用 Roles of IQGAP1 in Adjusting the Podocyte Cytoskeletop Reorganization

Keywords: IQGAP1,嘌呤霉素,足细胞,细胞骨架

Full-Text   Cite this paper   Add to My Lib

Abstract:

目的:研究支架蛋白IQGAP1在足细胞骨架重组中的作用。方法:用嘌呤霉素(PAN)刺激小鼠足细胞,实时定量PCR检测IQGAP1mRNA的表达,免疫印迹法检测足细胞IQGAP1蛋白的表达,激光共聚焦显微镜观察足细胞骨架F-actin分布,F-actin肌动蛋白评分系统(CFS)分析骨架重组;IQGAP1siRNA和IQGAP1质粒转染足细胞,分别观察下调和上调足细胞IQGAP1表达对嘌呤霉素诱导足细胞细胞骨架重组的改变。结果:嘌呤霉素刺激足细胞后IQGAP1mRNA和蛋白表达下调(P<0.05),足细胞F-actin分布发生明显重组;转染IQGAP1siRNA,嘌呤霉素刺激足细胞F-actin重组进一步加重;转染IQGAP1质粒,嘌呤霉素刺激足细胞F-actin重组减轻。结论:IQGAP1可能参与嘌呤霉素诱导的足细胞细胞骨架重组

References

[1]  Pollak MR.Inherited podocytopathies:FSGS and nephrotic syndrome from a genetic viewpoint[J].J Am Soc Nephrol,2002,13(12):3 016-3 023.
[2]  Barisoni L,Schnaper HW,Kopp JB.Advances in the biology and genetics of the podocytopathies:implications for diagnosis and therapy[J].Arch Pathol Lab Med,2009,133(2):201-216.
[3]  White CD,Erdemir HH,Sacks DB.IQGAP1and its binding proteins control diverse biological functions[J].Cell Signal,2012,24(4):826-834.
[4]  Lehtonen S,Ryan JJ,Kudlicka K,et al.Cell junctionassociated proteins IQGAP1,MAGI-2,CASK,spectrins,and alpha-actinin are components of the nephrin multiprotein complex[J].Proc Natl Acad Sci USA,2005,102(28):9 814-9 819.
[5]  Barisoni L,Schnaper HW,Kopp JB.A proposed taxonomy for the podocytopathies:a reassessment of the primary nephrotic diseases[J].Clin J Am Soc Nephrol,2007,2(3):529-542.
[6]  Lai LW,Yong KC,Lien YH.Site-specific expression of IQGAP1,a key mediator of cytoskeleton,in mouse renal tubules[J].J Histochem Cytochem,2008,56(7):659-666.
[7]  Ly J,Alexander M,Quaggin SE.A podocentric view of nephrology[J].Curr Opin Nephrol Hypertens,2004,13(3):299-305.
[8]  Smeets B,Te LN,Dijkman HB,et al.The parietal epithelial cell:a key player in the pathogenesis of focal segmental glomerulosclerosis in Thy-1.1 transgenic mice[J].J Am Soc Nephrol,2004,15(4):928-939.
[9]  Liu Y,Liang W,Yang Q,et al.IQGAP1mediates angiotensin II-induced apoptosis of podocytes via the ERK1/2 MAPK signaling pathway[J].Am J Nephrol,2013,38(5):430-444.
[10]  Attias O,Jiang R,Aoudjit L,et al.Rac1contributes to actin organization in glomerular podocytes[J].Nephron Exp Nephrol,2010,114(3):e93-e106.
[11]  Rigothier C,Auguste P,Welsh GI,et al.IQGAP1interacts with components of the slit diaphragm complex in podocytes and is involved in podocyte migration and permeability in vitro[J].PLoS One,2012,7(5):e37 695.
[12]  Schwarz K,Simons M,Reiser J,et al.Podocin,a raft-associated component of the glomerular slit diaphragm,interacts with CD2AP and nephrin[J].J Clin Invest,2001,108(11):1 621-1 629.
[13]  Mundel P,Reiser J,Zuniga MBA,et al.Rearrangements of the cytoskeleton and cell contacts induce process formation during differentiation of conditionally immortalized mouse podocyte cell lines[J].Exp Cell Res,1997,236(1):248-258.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133