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-  2018 

Fibulin-2调控的TGF-β/Smad信号通路在VSMCs凋亡中的作用 Function of Fibulin-2 Regulated TGF-Βeta/Smad Signaling Pathway in Vascular Smooth Muscle Cell Apoptosis

Keywords: Fibulin-2,转化生长因子-β1,血管平滑肌细胞,凋亡

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Abstract:

目的:了解腓骨蛋白-2(Fibulin-2,FBLN2)在急性Stanford A型主动脉夹层患者主动脉壁的表达情况并研究FBLN2调控的TGF-β/Smad信号通路在血管平滑肌细胞凋亡中的作用。方法:通过免疫组化染色和Western Blot检测急性Stanford A型主动脉夹层手术切除的主动脉标本FBLN2表达,并以重组FBLN2作用于体外培养的小鼠主动脉血管平滑肌细胞,通过Western Blot检测TGF-β1、Smad2/3和磷酸化Smad2/3的表达改变,同时使用A83-01阻断TGF-β1信号,观察平滑肌细胞凋亡情况。结果:FBLN2在急性Stanford A型主动脉夹层患者主动脉壁中表达显著升高(P<0.01),主要分布在主动脉中层。重组FBLN2可上调小鼠主动脉平滑肌细胞TGF-β1、pSmad2/3的表达(P<0.01),并抑制小鼠主动脉平滑肌细胞的凋亡(P<0.01)。当TGF-β1的作用被A83-01阻断时,重组FBLN2对主动脉平滑肌细胞凋亡的抑制作用显著减弱(P<0.05)。结论:FBLN2在急性Stanford A型主动脉夹层患者主动脉壁中层表达上调,并通过调控TGF-β/Smad信号通路抑制血管平滑肌细胞凋亡。FBLN2有可能参与急性Stanford A型主动脉夹层的发生

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