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-  2018 

溶酶体相关4次跨膜蛋白质β基因对前列腺癌细胞增殖和转移的影响 Downregulated Lysosome-Associated Protein Transmembrane 4 Beta Suppresses Tumor Cell Proliferation and Metastasis in Prostate Carcinoma

Keywords: LAPTM4B,前列腺癌,转染

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Abstract:

目的:研究溶酶体相关4次跨膜蛋白质β(LAPTM4B)基因在前列腺癌细胞中的表达水平和生物功能。方法:采用实时荧光定量PCR技术比较LAPTM4B在前列腺癌细胞中的表达差异。合成shRNA LAPTM4B质粒,用lipofectamine 2000介导转染前列腺癌细胞。RNA干扰技术下调LAPTM4B表达后,通过软琼脂细胞克隆形成实验、前列腺癌细胞侵袭和迁移实验研究前列腺癌细胞生物功能的改变。结果:LNCaP、DU145和PC-3细胞中LAPTM4BmRNA水平较RWPE-1明显增高,且差异具有统计学意义(P=0.001 2)。成功建立稳定转染shRNA LAPTM4B质粒和空白对照质粒的前列腺癌细胞株。LNCaP和PC-3细胞中LAPTM4BshRNA转染组细胞的体外克隆形成能力明显低于未转染组和空载体转染组,差异有统计学意义(P<0.000 1)。LAPTM4BshRNA转染组PC-3和LNCaP细胞到达对侧的迁移细胞数明显低于未转染组和空载体转染组,差异有统计学意义(P<0.05)。结论:LAPTM4B在前列腺癌细胞中表达上调,下调LAPTM4B在前列腺癌细胞中表达可以抑制前列腺癌细胞的增殖、侵袭和迁移能力

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