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-  2015 

埃兹蛋白在延迟着床小鼠子宫的表达及雌孕激素对其的调节作用 Expression of Ezrin in Mouse Endometrium Under Delayed Implantation and the Regulation of Ovarian Steroid Hormones on Its Expression

Keywords: 埃兹蛋白,小鼠,子宫内膜,雌激素,孕激素

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Abstract:

目的:探讨埃兹蛋白(Ezrin)在延迟着床小鼠子宫的表达及甾体激素对其表达的调节。方法:以昆明小鼠为实验对象,分别建立延迟着床与激活模型、小鼠卵巢切除类固醇激素处理模型,采用逆转录聚合酶链式反应(RT-PCR)法检测小鼠子宫内膜Ezrin mRNA的水平,免疫组织化学(IHC)ABC法检测小鼠子宫Ezrin的定位。结果:1延迟着床与激活小鼠子宫Ezrin的表达:RT-PCR结果显示,延迟着床激活组小鼠子宫着床点Ezrin的mRNA表达显著高于延迟着床组(P<0.05)。IHC结果显示,在延迟着床小鼠子宫腔上皮及基质中可检测到弱的Ezrin的免疫染色,在延迟着床激活组的小鼠子宫着床点,Ezrin的免疫染色主要位于着床位点下方的基质中。免疫组化染色半定量结果显示,延迟着床激活组小鼠子宫着床点Ezrin的蛋白水平较延迟着床组高(P<0.05)。2雌、孕激素对小鼠子宫Ezrin的表达调节:卵巢切除小鼠雌、孕激素处理后,芝麻油组未检测到Ezrin的mRNA或蛋白表达,而雌、孕激素均可诱导Ezrin的表达。RT-PCR结果显示,雌激素(E)组、孕激素(P)组和E+P组Ezrin的mRNA表达均升高(P<0.05),以E组更为显著(P<0.01),E+P组、P组和E组各组间Ezrin的mRNA表达有显著性差异(P<0.05)。IHC结果显示,芝麻油组Ezrin的免疫染色为阴性;E组Ezrin的免疫染色主要位于子宫内膜腔上皮和腺上皮;E+P组及P组在腔上皮、腺上皮和基质中均可检测到Ezrin蛋白的表达;半定量分析结果显示,与芝麻油组比较,E组、E+P组和P组小鼠子宫Ezrin的蛋白水平均升高(P<0.05),E组更为显著(P<0.01)。结论:推测小鼠子宫Ezrin的表达可能受雌孕激素的双重调节,雌激素可诱导小鼠子宫内膜上皮的Ezrin的表达,孕激素主要促进基质细胞Ezrin的表达

References

[1]  Dey SK,Lim H,Das SK,et al.Molecular cues to implantation[J].Endocr Rev,2004,25(3):341-373.
[2]  Curtis HS,Goulding EH,Eddy EM,et al.Studies using the estrogen receptor alpha knockout uterus demonstrate that implantation but not decidualization-associated signaling is estrogen dependent[J].Biol Reprod,2002,67(4):1 268-1 277.
[3]  Halon A,Donizy P.The role of the ERM protein family in maintaining cellular polarity,adhesion and regulation of cell motility[J].Postepy Hig Med Dosw(Online),2012,66:158-164.
[4]  Yoshinaga K,Adams CE.Delayed implantation in the spayed,progesterone treated adult mouse[J].Reprod Fertil,1966,12(3):593-599.
[5]  Mantena SR.C/EBP beta is a critical mediator of steroid hormone-regulated cell proliferation and differentiation in the uterine epithelium and stroma[J].Proc Natl Acad Sci USA,2006,103(6):1 870-1 875.
[6]  Lessey BA,Yeh I,Castelbaum,et al.Endometrial progesterone receptors and markers of urerine receptivity in the window of implantation[J].Fertil Sterile,1996,65(3):477-483.
[7]  Tan O,Ornek T,Fadiel A,et al.Expression and activation of the membrane-cytoskeleton protein ezrin during the normal endometrial cycle[J].Fertil Steril,2012,97(1):192-199.
[8]  McLaren A.A study of balstocysts during delay and subsequent implantation in lactating mice[J].Endocrinol,1968,42(3):453-463.
[9]  Smith PM,Heinrich CA,Pappas S,et al.Reciprocal regulation by estradiol 17-beta of Ezine and cadherincatenin complexes in pituitary GH(3)cells[J].Endocrine,2002,17(3):219-228.
[10]  Auvinen E,Carpen O,Korpela T,et al.Altered expression of ezrin,E-Cadherin and beta-Catenin in cervical neoplasia[J].Neoplasma,2013,60(1):56-61.
[11]  Cha J,Sun X,Dey SK.Mechanisms of implantation:strategies for successful pregnancy[J].Nat Med,2012,18(12):1 754-1 767.

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