全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...
-  2018 

HPV16 E6对ZEB1介导的宫颈癌细胞上皮间质转化的促进作用 Promoting Effect of HPV16 E6 on ZEB1-Mediated EMT in Cervical Cancer Cells

Keywords: 宫颈癌,HPV16 E6,E盒结合锌指蛋白1,上皮-间质转化

Full-Text   Cite this paper   Add to My Lib

Abstract:

目的:探讨宫颈癌细胞中HPV16E6病毒蛋白对E盒结合锌指蛋白1(ZEB1)及其介导的上皮间质转化(EMT)的影响。方法:构建HPV16E6质粒并转染至宫颈癌细胞C33A(E6-C33A组),同时设置Control-C33A组;设计合成靶向E6特异性shRNA,使Siha细胞中E6低表达(shE6-Siha组),设置Control-Siha组。划痕实验比较各组细胞迁移情况;荧光定量PCR和Western Blot检测EMT相关指标(E-cadherin、vimentin)及ZEB1的表达;生物信息学方法预测HPV16E6和ZEB1的三级结构及二者的相互作用,免疫共沉淀进行验证。结果:划痕实验显示E6-C33A组细胞迁移能力显著高于Control-C33A组;而shE6-Siha组细胞迁移能力显著低于Control-Siha组;与Control-C33A组相比,E6-C33A组中ZEB1、vimentin的表达量明显升高,E-cadherin表达则降低;与Control-Siha组相比,shE6-Siha组ZEB1、vimentin表达下降,E-cadherin表达升高。免疫共沉淀证明HPV16E6与ZEB1存在相互作用。结论:HPV16E6可促进ZEB1的表达,诱发宫颈癌细胞的EMT

References

[1]  La Vecchia C,Boccia S.Oral contraceptives,human papillomavirus and cervical cancer[J].Eur J Cancer Prev,2014,23(2):110-112.
[2]  Barquet-Munnoz SA,Rendon-Pereira GJ,Acunna-Gonzalez D,et al.Role of pelvic and para-aortic lymphadenectomy in abandoned radical hysterectomy in cervical cancer[J].World J Surg Oncol,2017,15(1):23.
[3]  Jedroszka D,Orzechowska M,Hamouz R,et al.Markers of epithelial-to-mesenchymal transition reflect tumor biology according to patient age and Gleason score in prostate cancer[J].PLoS One,2017,12(12):e0 188 842.
[4]  Geng F,Jiang Z,Song X,et al.Mdig suppresses epithelial-mesenchymal transition and inhibits the invasion and metastasis of nonsmall cell lung cancer via regulating GSK-3beta/beta-catenin signaling[J].International Journal of Oncology,2017,51(6):1 898-1 908.
[5]  Orellana-Serradell O,Herrera D,Castellon EA,et al.The transcription factor ZEB1promotes an aggressive phenotype in prostate cancer cell lines[J].Asian J Androl,2018,20(3):294-299.
[6]  Lee K,Gjorevski N,Boghaert E,et al.Snail1,Snail2,and E47promote mammary epithelial branching morphogenesis[J].EMBO J,2011,30(13):2 662-2 674.
[7]  Liu Y,Zhang N,Wang Y,et al.Zinc finger E-box binding homeobox 1promotes invasion and bone metastasis of small cell lung cancer in vitro and in vivo[J].Cancer Science,2012,103(8):1 420-1 428.
[8]  Pieters T,van Roy F.Role of cell-cell adhesion complexes in embryonic stem cell biology[J].J Cell Science,2014,127(Pt 12):2 603-2 613.
[9]  Xiong J,Wang D,Wei A,et al.Deregulated expression of miR-107inhibits metastasis of PDAC through inhibition PI3K/Akt signaling via caveolin-1and PTEN[J].Experimental Cell Research,2017,361(2):316-323.
[10]  Wang G,Lei L,Zhao X,et al.Calcitriol inhibits cervical cancer cell proliferation through downregulation of HCCR1expression[J].Oncology Research,2014,22(5-6):301-309.
[11]  Huang HJ,Zhou LL,Fu WJ,et al.beta-catenin SUMOylation is involved in the dysregulated proliferation of myeloma cells[J].Am J Cancer Res,2015,5(1):309-320.
[12]  Berx G,Raspe E,Christofori G,et al.Pre-EMTing metastasis?Recapitulation of morphogenetic processes in cancer[J].Clinical&Experimental Metastasis,2007,24(8):587-597.
[13]  Gao ZW,Wang HP,Dong K,et al.Adenosine inhibits migration,invasion and induces apoptosis of human cervical cancer cells[J].Neoplasma,2016,63(2):201-207.
[14]  Heldin CH,Vanlandewijck M,Moustakas A.Regulation of EMT by TGFbeta in cancer[J].FEBS Letters,2012,586(14):1 959-1 970.
[15]  Ran J,Lin DL,Wu RF,et al.ZEB1promotes epithelial-mesenchymal transition in cervical cancer metastasis[J].Fertil Steril,2015,103(6):1 606-1 614.e1-e2.
[16]  Chen Z,Li S,Huang K,et al.The nuclear protein expression levels of SNAI1and ZEB1are involved in the progression and lymph node metastasis of cervical cancer via the epithelial-mesenchymal transition pathway[J].Human Pathology,2013,44(10):2 097-2 105.
[17]  Li J,Khan MA,Wei C,et al.Thymoquinone inhibits the migration and invasive characteristics of cervical cancer cells SiHa and CaSki in vitro by targeting epithelial to mesenchymal transition associated transcription factors twist1and zeb1[J].Molecules,2017,22(12):pii:E2 105.
[18]  Zhang P,Sun Y,Ma L.ZEB1:at the crossroads of epithelial-mesenchymal transition,metastasis and therapy resistance[J].Cell Cycle,2015,14(4):481-487.
[19]  Tian B,Li X,Kalita M,et al.Analysis of the TGFbeta-induced program in primary airway epithelial cells shows essential role of NF-kappaB/RelA signaling network in typeⅡepithelial mesenchymal transition[J].BMC Genomics,2015,16:529.
[20]  Kaewprag J,Umnajvijit W,Ngamkham J,et al.HPV16oncoproteins promote cervical cancer invasiveness by upregulating specific matrix metalloproteinases[J].PLoS One,2013,8(8):e71 611.
[21]  Faghihloo E,Sadeghizadeh M,Shahmahmoodi S,et al.Cdc6expression is induced by HPV16E6and E7oncogenes and represses E-cadherin expression[J].Cancer Gene Ther,2016,doi:10.1038/cgt2016.51.[Epub ahead of print].
[22]  Mallini P,Lennard T,Kirby J,et al.Epithelial-tomesenchymal transition:what is the impact on breast cancer stem cells and drug resistance[J].Cancer Treatment Reviews,2014,40(3):341-348.
[23]  Ceppi P,Peter ME.MicroRNAs regulate both epithelial-to-mesenchymal transition and cancer stem cells[J].Oncogene,2014,33(3):269-278.
[24]  Vishnoi K,Mahata S,Tyagi A,et al.Human papillomavirus oncoproteins differentially modulate epithelial-mesenchymal transition in 5-FU-resistant cervical cancer cells[J].Tumour Biol,2016,37(10):13 137-13 154.
[25]  Voutsadakis IA.Epithelial-mesenchymal transition(EMT)and regulation of EMT factors by steroid nuclear receptors in breast cancer:A review and in silico investigation[J].J Clin Med,2016,5(1):pii:E11.
[26]  Li Y,Yu Y,Kang L,et al.Effects of chlorin e6-mediated photodynamic therapy on human colon cancer SW480cells[J].Int J Clin Exp Med,2014,7(12):4 867-4 876.
[27]  Kong J,Di C,Piao J,et al.Ezrin contributes to cervical cancer progression through induction of epithelialmesenchymal transition[J].Oncotarget,2016,7(15):19 631-19 642.
[28]  Sanchez-Tillo E,de Barrios O,Valls E,et al.ZEB1and TCF4 reciprocally modulate their transcriptional activities to regulate Wnt target gene expression[J].Oncogene,2015,34(46):5 760-5 770.
[29]  Brauksiepe B,Mujica AO,Herrmann H,et al.The Serine/threonine kinase Stk33exhibits autophosphorylation and phosphorylates the intermediate filament protein Vimentin[J].BMC Biochemistry,2008,9:25.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133