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-  2017 

一氧化碳释放分子2通过影响HMGB1分布及表达减轻心肌细胞缺氧损伤 CORM-2 Inhibits Myocardial Hypoxia Injury by Affecting the Distribution and Expression of HMGB1

Keywords: H9C2心肌细胞,CORM-2,缺氧,HMGB1,胞浆迁移

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Abstract:

目的:探讨一氧化碳释放分子(CORM-2)对心肌细胞缺氧的保护作用及其机制。方法:将大鼠心肌细胞H9C2分为4组:正常组(H9C2)、缺氧组(Hypoxia+H9C2)、CORM-2组(CORM-2+Hypoxia+H9C2)和iCORM-2组(iCORM-2+Hypoxia+H9C2)。CCK-8和流式细胞术分别检测心肌细胞活性和凋亡情况;免疫荧光检测HMGB1核浆分布情况;Western Blot检测心肌细胞胞浆内HMGB1表达和总HMGB1的表达情况,以及相关凋亡蛋白表达。结果:缺氧组与正常组比较,细胞活性下降,凋亡率增高(9.5%vs 27.4%,P<0.05),胞浆HMGB1迁移增加,细胞总HMGB1及凋亡相关蛋白表达增加(P<0.05)。CORM-2组与缺氧组相比,细胞的活性升高(P<0.05),凋亡率降低至14.1%,胞浆HMGB1释放量及总表达量减少,Cleaved Caspase-3表达降低,ICORM-2组与缺氧组无明显变化。结论:CORM-2通过影响HMGB1分布及表达减轻心肌细胞缺氧损伤

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