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-  2015 

人原发性胃非霍奇金淋巴瘤小鼠皮下移植瘤的病理学特性 Pathological Characteristics of Human Primary Gastric Non-Hodgkin's Lymphoma Subcutaneous Transplantation in Immunodeficient Mice

Keywords: 非霍奇金淋巴瘤,皮下移植,病理学

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Abstract:

目的:建立人原发性胃非霍奇金淋巴瘤小鼠皮下移植瘤模型,并探讨其组织病理学特性。方法:取新鲜的人原发性胃非霍奇金淋巴瘤组织移植于NOD/SCID小鼠和BALB/c-nu小鼠左、右肩胛部皮下,观察移植瘤的生长过程;待皮下移植瘤增大,将其取下传代,连续传至6代。同时每代移植瘤组织进行常规病理检测及CD20、CD45RB、Ki67的免疫组织化学检测。结果:在免疫缺陷小鼠体内形成了与人原发瘤形态结构一致的移植瘤,组织中见大量异常增生的大B淋巴细胞。免疫组织化学显示:CD20、CD45RB、Ki67阳性。结论:移植瘤较好地保留了人原发性胃非霍奇金淋巴瘤的组织病理学、生物学特性

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