全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...
-  2018 

超声联合靶向阳离子微泡介导Ang-1基因转染改善兔缺血心肌功能 Ultrasound Combined with Targeted Cationic Microbubbles Mediated Ang-1 Gene Transfection Improves the Cardiac Function of Rabbit with Myocardial Infarction

Keywords: 心肌梗死,靶向微泡,阳离子,超声,基因治疗

Full-Text   Cite this paper   Add to My Lib

Abstract:

目的:构建靶向阳离子微泡作为基因载体,增强超声靶向微泡破坏(UTMD)介导血管新生基因转染家兔心肌组织的效率,从而提高心肌梗死的疗效。方法:构建家兔心肌梗死模型,制备细胞间黏附分子(ICAM)-1靶向阳离子微泡(TCMB)和非靶向普通阳离子微泡(CMB),以生理盐水为空白对照(Control),将家兔分为3组:TCMB治疗组,CMB治疗组和Control组,在UTMD作用下分别介导血管生成素-1(Ang-1)基因转染家兔心肌组织,比较三组转染效率及心功能。结果:CMB与TCMB的粒径无明显差异[(2.9±1.9)μmvs(3.1±2.1)μm,P>0.05],二者表面电位分别为(+31.3±3.9)mV和(+22.0±2.4)mV(P<0.01),每千万个微泡的载基因量分别为(3.3±0.7)μg和(4.0±0.9)μg(P<0.05)。转染治疗后3d,TCMB组的Ang-1基因表达高于CMB组,CMB组高于Control组(均P<0.05)。与心梗后2d相比,转染治疗后28d,Control组左室射血分数LVEF进一步减低[(44±10)%],而CMB组和TCMB组均有所改善,但以TCMB组显著[(59±7)%vs(54±8)%,P<0.05]。结论:ICAM-1靶向阳离子微泡能增加载基因能力及靶向性,提高UTMD介导Ang-1基因转染的效率,改善兔缺血心肌功能

References

[1]  Susanne R,Christian T,Christian H,et al.Ischemia and reperfusion related myocardial inflammation:A network of cells and mediators targeting the cardiomyocyte[J].IUBMB Life,2015,67(2):110-119.
[2]  Moreira DM,da Silva RL,Vieira JL,et al.Inflammation and anti-inflammatory drugs in coronary artery disease[J].Am J Cardiovasc Drugs,2015,15(1):1-11.
[3]  邓倾,王益佳,周青,等.血管内皮靶向微泡对兔心肌缺血再灌注损伤的超声分子显像[J].微循环学杂志,2017,27(4):18-23.Deng Q,Wang YJ,Zhou Q,et al.Vascular endothelium-targeted microbubbles for the target ultrasound imaging of myocardial ischemia reperfusion injury in rabbit[J].Chinese Journal of Microcirculation,2017,27(4):18-23.
[4]  Scimia MC,Gumpert AM,Koch WJ.Cardiovascular gene therapy for myocardial infarction[J].Expert Opin Biol Ther,2014,14(2):183-195.
[5]  Liao YY,Chen ZY,Wang YX,et al.New progress in angiogenesis therapy of cardiovascular disease by ultrasound targeted microbubble destruction[J].Biomed Res Int,2014,2014:872984.
[6]  Delalande A,Postema M,Mignet N,et al.Ultrasound and microbubble-assisted gene delivery:recent advances and ongoing challenges[J].Ther Deliv,2012,3(10):1 199-1 215.
[7]  Tzu-Yin W,Wilson KE,Machtaler S,et al.Ultrasound and microbubble guided drug delivery:mechanistic understanding and clinical implications[J].Curr Pharm Biotechnol,2014,14(8):743-752.
[8]  Sahdev P,Ochyl LJ,Moon JJ.Biomaterials for nanoparticle vaccine delivery systems[J].Pharm Res,2014,31(10):2 563-2 582.
[9]  Sun L,Huang CW,Wu J,et al.The use of cationic microbubbles to improve ultrasound-targeted gene delivery to the ischemic myocardium[J].Biomaterials,2013,34(8):2 107-2 116.
[10]  周青,陈鹏,邓倾,等.携抗细胞间黏附分子-1微泡对心肌梗死受损内皮靶向识别的实验研究[J].武汉大学学报:医学版,2014,35(3):329-332.Zhou Q,Chen P,Deng Q,et al.Experimental study on targeted recognition of microbubbles carrying ICAM-1antibody to injuried endothelium after myocardial infarction[J].Medical Journal of Wuhan University,2014,35(3):329-332.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133