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-  2018 

miR-22调控VASP抑制胃癌细胞增殖和侵袭 MiR-22 Targeting VASP Inhibits Gastric Cancer Cell Proliferation and Invasion

Keywords: MiR-22,VASP,胃癌,增殖,侵袭

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Abstract:

目的:探讨miR-22调控胃癌细胞增殖、侵袭的机制。方法:平板克隆实验、CCK-8、Transwell实验检测miR-22表达水平对胃癌细胞克隆形成、增殖、侵袭能力的影响。实时荧光定量PCR、蛋白印迹法检测miR-22表达水平对胃癌细胞中血管扩张刺激磷蛋白(VASP)表达的影响。结果:过表达miR-22显著抑制胃癌细胞BGC-823的克隆形成、增殖及侵袭能力。过表达miR-22后,胃癌细胞中VASP的mRNA及蛋白表达水平显著降低。结论:在胃癌细胞BGC-823中miR-22表达与VASP的表达显著负相关。miR-22通过抑制VASP的表达从而进一步抑制胃癌细胞的增殖和侵袭

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