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-  2017 

细胞凋亡在脑死亡状态致肺脏损伤中的作用 The Role of Apoptosis in Lung Injury Induced by Brain Death

Keywords: 脑死亡,肺移植,Bax,Caspase 3,细胞凋亡

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Abstract:

目的:观察兔脑死亡后不同时间点肺组织的细胞凋亡情况和相关基因的表达变化,探究脑死亡状态致肺损伤发生的分子机制。方法:40只健康家兔随机数表法分为2组,假手术组(n=20):行气管插管、股动脉插管及钻孔开颅手术,置入Foley 3F气囊导管后不加压;脑死亡组(n=20):成功建立兔脑死亡模型。各组在术后2,6及8h记录动脉血压和心率变化,RT-PCR检测各组Bcl-2相关X蛋白(Bax)的mRNA表达,免疫组织化学法检测肺组织caspase 3的表达,并用TUNEL法检测肺组织的细胞凋亡情况。结果:脑死亡后2,6,8h家兔动脉血压、心率差异无统计学意义(P>0.05)。脑死亡组各时间点caspase 3阳性表达细胞数较假手术组均有升高(P<0.05)。TUNEL染色显示,脑死亡组细胞凋亡指数在6h和8h达到80%,提示肺损伤逐渐加重,这与促凋亡基因Bax mRNA表达的趋势一致。结论:脑死亡状态可导致肺脏损伤,随着脑死亡时间的延长逐渐加重,且该变化与相关凋亡基因的过表达有关。脑死亡状态维持6h以上,肺脏细胞凋亡情况最为严重

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