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-  2015 

核受体GCNF对H9C2心肌细胞的影响及作用机制 Effects and Mechanisms of Human Germ Cell Nuclear Factor in H9C2 Cells

Keywords: 生殖细胞核因子,H9C2,表达载体,凋亡,RIPK2,RIPK3

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Abstract:

目的:研究人孤儿核受体生殖细胞核因子(hGCNF)对H9C2心肌细胞的影响并初探其机制。方法:通过感染Ad-hGCNF腺病毒使H9C2中的GCNF基因表达上调,利用间接免疫荧光化学技术确定其亚细胞定位,通过MTT法研究GCNF表达对细胞活力的影响,DAPI染色检测细胞凋亡水平,实时定量PCR及Western blot技术检测受体结合蛋白激酶2(RIPK2)和受体结合蛋白激酶3(RIPK3)的表达水平。结果:Ad-hGCNF腺病毒感染后,H9C2心肌细胞GCNF表达上调,亚细胞定位位于细胞核,细胞活力降低,凋亡水平显著增加,RIPK2和RIPK3表达水平均升高。结论:感染Ad-hGCNF腺病毒表达载体降低H9C2细胞活力,增加其凋亡水平,机制与RIPK2和RIPK3的表达上调有关

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