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-  2017 

hsa-miR-214-3p对宫颈癌SiHa细胞生物学行为的影响 Effect of hsa-miR-214-3p on the Biological Behavior of Cervical Cancer SiHa Cells

Keywords: 宫颈癌,hsa-miR-214-3p,增殖,凋亡,迁移

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Abstract:

目的:探讨hsa-miR-214-3p对宫颈癌SiHa细胞生物学行为的影响以及可能的作用机制。方法:将hsamiR-214-3p mimics/inhibitor转染SiHa细胞,分析过表达和下调内源性hsa-miR-214-3p的表达对SiHa细胞增殖、迁移、凋亡的影响。用TargetScanHuman7.1、Pictar、miRanda、miRTarBase以及DAVID数据库进行靶基因预测和富集通路分析。结果:hsa-miR-214-3p在宫颈癌细胞中呈高表达;过表达hsa-miR-214-3p后,细胞的增殖和克隆形成能力明显增强(P<0.05);而当抑制内源性hsa-miR-214-3p的表达后,细胞的增殖能力则明显减弱(P<0.001),流式细胞仪检测细胞凋亡结果显示SiHa细胞的凋亡比例明显上升(P<0.05),Western Blot的结果显示Bcl2蛋白的表达显著下降(P<0.05);过表达hsa-miR-214-3p和抑制内源性hsa-miR-214-3p表达对SiHa细胞的迁移均没有显著的影响(P>0.05);生物信息学分析结果显示,hsa-miR-214-3p的靶基因主要富集在磷脂酰肌醇信号系统以及Wnt信号通路等。结论:过表达hsa-miR-214-3p可以促进宫颈癌细胞的增殖;抑制内源性hsa-miR-214-3p的表达可以抑制SiHa细胞的增殖并促进细胞凋亡;hsa-miR-214-3p可能通过调节Bcl2基因的表达来调控SiHa细胞的凋亡进程。上述结果为宫颈癌分子机制的研究提供了新线索,同时也为宫颈癌的临床诊断与治疗提供潜在靶点

References

[1]  Phatak P,Byrnes KA,Mansour D,et al.Overexpression of miR-214-3p in esophageal squamous cancer cells enhances sensitivity to cisplatin by targeting survivin directly and indirectly through CUG-BP1[J].Oncogene,2016,35(16):2 087-2 097.
[2]  Xu J,Wan X,Chen X,et al.miR-2861acts as a tumor suppressor via targeting EGFR/AKT2/CCND1 pathway in cervical cancer induced by human papillomavirus virus 16E6[J].Sci Rep,2016,6:28968.
[3]  Wang X,Chen J,Li F,et al.MiR-214inhibits cell growth in hepatocellular carcinoma through suppression ofβ-catenin[J].Biochem Biophys Res Commun,2012,428(4):525-531.
[4]  Liu Y,Zhou H,Ma L,et al.MiR-214suppressed ovarian cancer and negatively regulated semaphorin 4D[J].Tumour Biol,2016,37(6):8 239-8 248.
[5]  Chen W,Zheng R,Baade PD,et al.Cancer statistics in China,2015[J].CA Cancer J Clin,2016,66(2):115-132.
[6]  Hayes J,Peruzzi PP,Lawler S.MicroRNAs in cancer:biomarkers,functions and therapy[J].Trends Mol Med,2014,20(8):460-469.
[7]  Wang F,Lv P,Liu X,et al.microRNA-214enhances the invasion ability of breast cancer cells by targeting p53[J].Int J Mol Med,2015,35(5):1 395-1 402.
[8]  Mohr AM,Mott JL.Overview of microRNA biology[J].Semin Liver Dis,2015,35(1):3-11.
[9]  徐战战,石芳,赵旻.Let-7i抑制人卵巢癌SKOV3细胞的增殖、迁移和侵袭[J].肿瘤,2016,36(2):149-157.Xu Z,Shi F,Zhao M.Let-7iinhibits the proliferation,migration and invasion of human ovarian cancer Skov3cells[J].Tumor,2016,36(2):149-157.
[10]  Peralta-Zaragoza O,Deas J,Meneses-Acosta A,et al.Relevance of miR-21in regulation of tumor suppressor gene PTEN in human cervical cancer cells[J].BMC Cancer,2016,16:215.
[11]  Li J,Hu L,Tian C,et al.microRNA-150promotes cervical cancer cell growth and survival by targeting FOXO4[J].BMC Mol Biol,2015,16:24.
[12]  Zhu MY,Chen F,Niyazi M,et al.Variation in apoptotic gene expression in cervical cancer through oligonucleotide microarray profiling[J].J Low Genit Tract Dis,2015,19(1):46-54.
[13]  Hata AN,Engelman JA,Faber AC.The BCL2family:key mediators of the apoptotic response to targeted anticancer therapeutics[J].Cancer Discov,2015,5(5):475-487.
[14]  Xin R,Bai F,Feng Y,et al.MicroRNA-214promotes peritoneal metastasis through regulating PTEN negatively in gastric cancer[J].Int Heart J,2016,57(2):247-250.
[15]  Deng M,Ye Q,Qin Z,et al.miR-214promotes tumorigenesis by targeting lactotransferrin in nasopharyngeal carcinoma[J].Tumour Biol,2013,34(3):1 793-1 800.

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