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-  2018 

蛇床子素对棕榈酸诱导的NIT-1细胞损伤的影响 Protective Effect of Osthole on Injury Induced by Palmitic Acid in Pancreatic Beta Cells

Keywords: 蛇床子素,糖尿病,胰岛β细胞,凋亡

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Abstract:

目的:研究蛇床子素在棕榈酸(PA)诱导下对于NIT-1细胞的保护作用。方法:将NIT-1细胞分为空白组、PA组、PA+蛇床子素组,培养3d后,置于流式细胞仪测定凋亡率,通过RT-PCR测定脂代谢相关基因PPARα、UCP-2和内质网应激相关基因CHOP及GRP78mRNA的表达,并通过Western Blot测定凋亡相关蛋白Caspase3、Bax、Bcl-2和内质网应激相关蛋白eIF2α、p-eIF2α的表达。结果:蛇床子素能降低棕榈酸诱导的细胞凋亡率,增加PPARα、UCP-2mRNA的表达,降低CHOP及GRP78mRNA的表达。另外,蛇床子素能够使凋亡相关蛋白Caspase3、Bax的表达下降,抗凋亡蛋白Bcl-2蛋白增加,并降低eIF-2α的磷酸化水平。结论:蛇床子素对棕榈酸诱导的NIT-1细胞损伤有保护作用,这种保护作用可能和PPARα和内质网应激通路有关

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