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-  2018 

Tiam1和Rac1蛋白在肾细胞癌中的表达及其与临床病理特征之间的关系 Tiam1 and Rac1 Expression in Renal Cell Carcinoma and Their Relation to Clinical-Pathological Features

Keywords: 肾细胞癌,Tiam1,Rac1,病理特征,量子点,免疫组织化学

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Abstract:

目的:研究肾细胞癌中T淋巴瘤浸润和转移诱导因子1(Tiam1)和Ras相关的C3肉毒素底物1(Rac1)蛋白的表达与肿瘤病理分型、分级和侵袭转移的关系。方法:收集武汉大学人民医院病理科2008-2014年有完整临床和病理资料的存档蜡块60例肾细胞癌(42例肾透明细胞癌,12例乳头状肾细胞癌,6例肾嫌色细胞癌)及10例癌旁肾组织。采用新的标记试剂——量子点(QDs)免疫荧光组织化学技术检测60例肾细胞癌和10例癌旁肾组织中Tiam1和Rac1蛋白的表达水平。分析Tiam1和Rac1蛋白的表达与肾细胞癌的临床和病理特征的关系。应用HPIAS-1000高清晰度彩色病理图文报告管理系统,对Tiam1和Rac1蛋白的表达进行定量分析,并用SPSS 15.0软件对各组免疫荧光组织化学反应阳性颗粒的吸光度值、阳性面积率做单因素方差分析和SNK(q)检验。并采用Spearman等级相关检验,分析Tiam1和Rac1蛋白反应阳性颗粒的吸光度值、阳性面积率表达的相关性。结果:(1)量子点(QDs)免疫荧光组织化学染色Tiam1和Rac1蛋白在肾细胞癌中呈高表达,而在癌旁组织中呈低表达。肾细胞癌与癌旁组织相比,差异有显著性(P<0.05);(2)Tiam1和Rac1蛋白表达水平与患者性别、年龄及肿瘤大小均没有相关性(P>0.05),但与肿瘤的分化程度、淋巴结转移及分期均有相关性(P<0.05)。(3)Tiam1和Rac1蛋白在肾细胞癌组织中表达的相关性经Spearman等级相关分析显示,Tiam1和Rac1蛋白表达呈正相关(r=0.425,P<0.05)。结论:(1)Tiam1和Rac1蛋白在肾细胞癌中的表达异常可能是肾细胞癌的早期分子事件,提示二者可能均具有原癌基因的功能,均具有致瘤活性。(2)Tiam1和Rac1蛋白在肾细胞癌中的高表达与肿瘤淋巴结转移、分化程度及分期有关,提示Tiam1和Rac1蛋白在肾细胞癌侵袭转移中发挥重要作用。(3)Tiam1和Rac1蛋白在肾细胞癌的表达是呈正相关的,有可能存在内在的必然的联系。提示它们在肾细胞癌的发生、发展过程中相互作用、相互调控,两者起着协同作用

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