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-  2018 

凋亡显像剂~(99)mTc(CO)_3-Annexin V的制备及其在小鼠体内的生物学分布 Preparation of Apoptosis Imaging Agent ~(99m)Tc(CO)_3-Annexin V and Its Biodistribution in Mice

Keywords: Annexin V,三羰基锝,凋亡显像,昆明小鼠,生物分布

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Abstract:

目的:使用放射性核素~(99m)Tc标记特异性靶向凋亡蛋白Annexin V,并研究标记化合物在小鼠体内的药代动力学特点。方法:采用Tricarbonyl kit制备标记前体三羰基锝化合物盐溶液以标记Annexin V;测定标记化合物的标记率、放化纯及稳定性。通过生物分布实验研究~(99m)Tc(CO)_3-Annexin V在健康昆明小鼠体内的药代动力学特点。结果:成功制备了~(99m)Tc(CO)_3-Annexin V,标记率为(57.21±2.83)%,比活度为(5.65±0.43)mCi/100μg,放化纯>90%,其在体外稳定性良好,至标记后6h放化纯仍大于>90%。~(99m)Tc(CO)_3-Annexin V在小鼠体内循环半衰期较短,注入体内2h后即可排出约40%,4h后排出约60%。肾脏和肝脏是~(99m)Tc(CO)_3-Annexin V的主要排泄器官。结论:本研究表明利用Tricarbonyl kit可以成功将放射性核素~(99m)Tc标记Annexin V,标记率高且方法简便可行,是一种具有广泛应用前景的凋亡显像剂

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