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-  2018 

激动α7nΑCHRs对脓毒症小鼠血小板的影响 Effect of Activating the Alpha7 Nicotinic Acetylcholine Receptors on Platelet in Septic Mice

Keywords: 脓毒症,血小板,α7亚基烟碱型乙酰胆碱受体

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Abstract:

目的:观察激动α7亚基烟碱型乙酰胆碱受体(α7nΑCHRs)对脓毒症小鼠血小板的影响。方法:将30只雄性C57BL/6小鼠按随机数字表法随机分为3组:假手术组(sham组)、盲肠结扎+穿孔组(CLP组)、CLP+α7nΑCHRs激动组(CLP+GTS-21组),每组10只。于模型制备成功后24h采血,检测血小板计数和血小板表面CD62P的表达。结果:血小板计数:CLP组血小板计数较sham组明显下降(P<0.05),CLP+GTS-21组血小板下降程度明显减轻(P<0.05),但CLP+GTS-21组血小板计数仍低于sham组(P<0.05)。血小板CD62P的表达:sham组、CLP组、CLP+GTS-21组血小板CD62P的表达均无明显差异(P>0.05)。结论:脓毒症小鼠会发生明显的血小板减少,激动α7nΑCHRs可以缓解脓毒症小鼠血小板减少,检测血小板CD62P的表达水平不能反映脓毒症小鼠血小板的活化

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