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-  2015 

去除尼古丁和焦油的香烟烟雾提取物与尼古丁对大鼠睾丸氧化应激损伤及睾酮影响的比较 Comparison of Nicotine-and Tar-Free Cigarette Smoke Extract and Nicotine in Testis Injury on Oxidative Stress and Plasma Testosterone Level in Rats

Keywords: 去除尼古丁和焦油的香烟烟雾提取物,尼古丁,睾酮,氧化应激

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Abstract:

目的:探讨去除尼古丁和焦油的香烟烟雾提取物(CSE)与尼古丁对大鼠睾丸氧化应激损伤及睾酮影响的差异及其机制。方法:30只SD雄性大鼠随机分为3组:对照组(PBS组)、CSE组及尼古丁组。8周后放射免疫法检测血清睾酮(T)、卵泡刺激素(FSH)、黄体生成素(LH)水平;用黄嘌呤氧化酶法测定超氧化物歧化酶(SOD)活性、硫代巴比妥酸法测定丙二醛(MDA)活性;HE染色观察睾丸组织形态学变化,并用电镜观察睾丸间质细胞和精子细胞的超微结构变化。结果:HE染色显示尼古丁组生精小管萎缩,生精细胞排列紊乱,数量减少。电镜下尼古丁组精子细胞出现异型性、凋亡,CSE组睾丸间质细胞中出现线粒体水肿,线粒体嵴消失及溶酶体增多。CSE、尼古丁组T值[(2.58±0.81)ng/ml,(1.74±0.51)ng/ml]、SOD值[(28.28±1.27)U/mg prot,(26.42±2.02)U/mg prot]较PBS组[T(6.02±0.75)ng/ml,SOD(38.07±1.39)]显著降低(P<0.05),MDA值[(0.65±0.070)U/mg prot,(0.76±0.073)U/mg prot]较PBS组[(0.50±0.066)U/mg prot]显著升高(P<0.05),两组之间比较有显著性差异(P<0.05),且T值与MDA呈负相关,与SOD呈正相关。对照组和CSE组FSH[(1.97±0.25),(1.92±0.29)mIU/ml]、LH[(8.49±0.42),(8.14±0.46)mIU/ml]显著高于尼古丁组[FSH(1.54±0.28)mIU/ml,LH(7.01±0.61)mIU/ml](P<0.05),但两组之间比较差异无统计学意义(P>0.05)。结论:CSE与尼古丁对睾丸的损伤及血清睾酮的影响与氧化应激有关,但是两者对睾丸损伤部位、性激素及氧化应激水平影响不一

References

[1]  Lee SY,Gong EY,Hong CY,et al.ROS inhibit the expression of testicular steroidogenic enzyme genes via the suppression of Nur77transactivation[J].Free Radic Biol Med,2009,47(11):1 591-1 600.
[2]  占习双,吴天鹏,欧阳海,等.香烟烟雾对雄性大鼠性功能影响的实验研究[J].中华男科学杂志,2009,15(9):788-791.
[3]  农谦,吴天鹏,欧阳海.香烟烟雾致睾丸氧化应激损伤与iNOS表达的关系[J].武汉大学学报:医学版,2008,29(3):312-314.
[4]  Tostes RC,Carneiro FS,Lee AJ,et al.Cigarette smoking and erectile dysfunction:focus on NO bioavailability and ROS generation[J].J Sex Med,2008,5(6):1 284-1 295.
[5]  Slotkin TA.Fetal nicotine or cocaine exposure:which one is worse[J]?J Pharmacol Exp Ther,1998,285(3):931-945.
[6]  Park MG,Ko KW,Oh MM,et al.Effects of smoking on plasma testosterone level and erectile function in rats[J].J Sex Med,2012,9(2):472-481.
[7]  Halmenschlager G,Rossetto S,Lara GM,et al.Evaluation of the effects of cigarette smoking on testosterone levels in adult men[J].J Sex Med,2009,6(6):1 763-1 772.
[8]  Oyeyipo IP,Raji Y,Bolarinwa AF.Nicotine alters male reproductive hormones in male albino rats:The role of cessation[J].J Hum Reprod Sci,2013,6(1):40-44.
[9]  Jeng HA,Chen YL,Kantaria KN.Association of cigarette smoking with reproductive hormone levels and semen quality in healthy adult men in Taiwan[J].J Environ Sci Health A Tox Hazard Subst Environ Eng,2014,49(3):262-268.
[10]  Asano H,Horinouchi T,Mai Y,et al.Nicotine-and tar-free cigarette smoke induces cell damage through reactive oxygen species newly generated by PKC-dependent activation of NADPH oxidase[J].J Pharmacol Sci,2012,118(2):275-287.
[11]  Mitra A,Chakraborty B,Mukhopadhay D,et al.Effect of smoking on semen quality,FSH,testosterone level,and CAG repeat length in androgen receptor gene of infertile men in an Indian city[J].Syst Biol Reprod Med,2012,58(5):255-262.
[12]  Shimosato T,Geddawy A,Tawa M,et al.Chronic administration of nicotine-free cigarette smoke extract impaired endothelium-dependent vascular relaxation in rats via increased vascular oxidative stress[J].J PharmacolSci,2012,118(2):206-214.

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