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-  2015 

冬凌草甲素对棕榈酸诱导的内皮细胞损伤的保护作用 Protection of Oridonin on Endothelial Cells from Injury Induced by Palmitic Acid

Keywords: 冬凌草甲素,血管内皮细胞,棕榈酸,一氧化氮

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Abstract:

目的:探讨冬凌草甲素(oridonin)对棕榈酸诱导的人脐静脉内皮细胞(HUVECs)损伤的保护作用。方法:体外培养HUVECs,噻唑蓝法(MTT)确定冬凌草甲素作用的最佳浓度和时间;细胞分为空白对照组、棕榈酸组、棕榈酸+oridonin预处理组(以下简称"oridonin预处理组",棕榈酸浓度为500μmol/L),oridonin预处理24h,棕榈酸作用48h。流式细胞仪检测细胞凋亡率,硝酸还原酶法检测各组细胞上清液中一氧化氮(NO)的浓度。结果:冬凌草甲素作用于HUVECs细胞的最佳时间是24h,最佳浓度为5μmol/L。与空白对照组比较,棕榈酸组HUVECs细胞凋亡率[(21.23±4.11)%vs(1.63±1.23)%,t=7.90,P<0.01]明显增加,上清液中NO的浓度[(114.67±17.01)μmol/Lvs(242.33±20.11)μmol/L,t=-8.396,P<0.01]明显降低。相对于棕榈酸组,oridonin预处理组HUVECs凋亡率明显降低[(7.77±2.57)%vs(21.23±4.11)%,t=-4.81,P<0.01],上清液中NO的浓度[(191.33±13.01)μmol/L vs(114.67±17.01)μmol/L,t=6.20,P<0.01]明显升高。结论:冬凌草甲素能抑制棕榈酸诱导的HUVECs细胞凋亡,并增加NO的浓度

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