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-  2018 

大鼠皮肤伤口感染模型的建立 Establishment of Skin Wound Infection Model in Rats

Keywords: 大鼠,皮肤伤口,金黄色葡萄球菌,大肠杆菌,感染模型

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Abstract:

目的:探讨大鼠皮肤伤口大肠杆菌(E.coli)和金黄色葡萄球菌(S.aureus)感染模型的制备方法,建立大鼠背部皮肤伤口感染的模型,为临床创伤及术后伤口感染愈合提供小动物研究模型,同时为新型生物医学敷料及药物等的研究提供模型基础。方法:剃除大鼠背部毛发,同时用7%硫化钠溶液对大鼠背部剩余的毛发彻底清除。于大鼠脊柱两侧分别剪取直径为1.5cm的伤口,保证伤口深达肌层。将实验分为3组:(1)非感染组;(2)E.coli感染组;(3)S.aureus感染组。在大鼠行伤口制备术后,按分组将100μl生理盐水或菌液滴加到相应组别伤口表面。在伤口表面贴附临床静脉留置针贴膜,同时在贴膜表面加以医用胶布固定。术后每只大鼠于独立通气笼具(IVC笼具)内饲养,24-48h后观察各组伤口表面感染情况。结果:术后48h伤口处可形成明显的感染灶,E.coli菌液浓度在109集落形成单位(CFU)/ml以内可形成明显感染且不致死,而S.aureus感染伤口菌液浓度在超过10~8 CFU/ml后感染严重且致死率较高,菌液浓度控制在106-10~8 CFU/ml之间能够形成明显的S.aureus感染灶且能保证大鼠存活。结论:S.aureus菌液(10~6-10~7 CFU/ml,100μl)、E.coli菌液浓度(107-10~8 CFU/ml,100μl)能够成功制备大鼠伤口感染模型,且保证大鼠正常存活

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