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-  2015 

细胞因子CCL25对鼻咽癌CNE-2细胞迁移的影响 Effect of Chemokine CCL25 on CNE-2 Cell Migration

Keywords: 趋化性细胞因子,趋化性细胞因子受体,鼻咽癌,CNE-2细胞

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Abstract:

目的:研究CCL25是否影响鼻咽癌CNE-2细胞的迁移。方法:采用Transwell实验,上室中加入CNE-2细胞,下室中加入不同浓度的CCL25,观察其对CNE-2向下室迁移的影响。另外同时在下室中加入anti-CCR9,再观察CNE-2向下室的迁移情况。结果:CCL25具有趋化CNE-2细胞的作用,同空白对照组相比,50ng/ml的CCL25能明显增强CNE-2细胞的迁移率(P<0.01)。而1μg/ml的anti-CCR9能逆转CCL25对CNE-2细胞的促迁移作用(P<0.05)。结论:CCL25具有趋化CNE-2细胞,促进CNE-2细胞迁移的作用。Anti-CCR9能阻断CCL25对CNE-2细胞的促迁移作用

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