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-  2015 

人足月胎盘滋养细胞分离培养方法的改良 Improved Method for Isolating and Culturing Term Human Placental Trophoblasts

Keywords: 人足月胎盘,滋养细胞,原代培养

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Abstract:

目的:建立一种经济、高效的人足月胎盘滋养细胞分离培养方法。方法:收取正常足月剖宫产患者胎盘组织,DNA酶联合胰酶反复消化、网筛过滤、不连续密度梯度分离液分离纯化细胞;免疫细胞化学法鉴定细胞来源;对所培养的滋养细胞进行形态学观察;CCK-8增殖实验分析细胞体外增殖活力。结果:利用此法可成功体外培养胎盘滋养细胞,一次操作可获得大于107个细胞。体外培养时,滋养细胞增殖能力低下,3h开始贴壁,24h后逐渐开始融合成合体滋养细胞。结论:本研究改进了既往足月胎盘滋养细胞原代培养的方法,简化操作步骤,缩短操作时间,并且一次性可获得大量且纯度较好的胎盘滋养细胞

References

[1]  Zhou WH,Du MR,Dong L.Chemokine CXCL12promotes the cross-talk between trophoblasts and decidual stromal cells in human first-trimester pregnancy[J].Hum Reprod,2008,23(12):2 669-2 679.
[2]  Wu X,Li DJ,Yuan MM.The expression of CXCR4/CXCL12in first-trimester human trophoblast cells[J].Biol Reprod,2004,70(6):1 877-1 885.
[3]  Borbely AU,Sandri S,Fernandes IR,et al.The term basal plate of the human placenta as a source of functional extravillous trophoblast cells[J].Reprod Biol Endocrinol,2014,12:7.
[4]  Petroff MG,Phillips TA,Ka H,et al.Isolation and culture of term human trophoblast cells[J].Methods Mol Med,2006,121:203-217.
[5]  Potgens AJ,Kataoka H,Ferstl S,et al.A positive immunoselection method to isolate villous cytotrophoblast cells from first trimester and term placenta to high purity[J].Placenta,2003,24(4):412-423.
[6]  Aplin JD,Jones CJ,Harris LK.Adhesion molecules in human trophoblast——a review.Ⅰ.Villous trophoblast[J].Placenta,2009,30(4):293-298.
[7]  张九聪,孙利,聂青和.人胎盘滋养细胞原代培养与改进[J].生物技术通讯,2010,21(1):58-61.
[8]  Ren L,Liu YQ,Zhou WH.Trophoblast-derived chemokine CXCL12promotes CXCR4expression and invasion of human first-trimester decidual stromal cells[J].Hum Reprod,2012,27(2):366-374.
[9]  Aplin JD.Developmental cell biology of human villous trophoblast:current research problems[J].Int J Dev Biol,2010,54(2-3):323-329.
[10]  Kliman HJ,Nestler JE,Sermasi E,et al.Purification,characterization,and in vitro differentiation of cytotrophoblasts from human term placentae[J].Endocrinology,1986,118(4):1 567-1 582.
[11]  肖敏,凌斌,姚凤球,等.人胎盘滋养层细胞分离和鉴定[J].中国妇幼保健,2008,23(18):2 567-2 569.
[12]  王冬菊,肖小敏,肖昕.人足月胎盘滋养细胞分离纯化与鉴定[J].实用儿科临床杂志,2010,25(17):1 353-1 356.
[13]  Knofler M.Critical growth factors and signalling pathways controlling human trophoblast invasion[J].Int J Dev Biol,2010,54(2-3):269-280.
[14]  Miller J,Turan S,Baschat AA.Fetal growth restriction[J].Semin Perinatol,2008,32(4):274-280.

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