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-  2017 

抑制泛素特异性蛋白酶9X表达对胶质瘤U251细胞生长及凋亡的影响 Effect of Low Expression of USP9X on the Growth and Apoptosis of Glioma U251 Cells

Keywords: 泛素特异性蛋白酶(9X),胶质瘤,细胞凋亡

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Abstract:

目的:研究抑制泛素特异性蛋白酶(USP)9X对胶质瘤U251细胞生长及凋亡的影响,并探讨其可能的机制。方法:CCK-8法检测抑制USP9X表达对U251细胞生长的影响,流式细胞术检测抑制USP9X对U251细胞凋亡的影响,Western Blot法检测USP9X对U251细胞蛋白水平的调控。结果:抑制USP9X表达对U251细胞生长有显著抑制作用;与对照组相比,实验组U251细胞凋亡率上升,实验组β-catenin蛋白的表达较对照组下调。结论:抑制USP9X表达可以抑制胶质瘤U251细胞的生长,促进细胞凋亡,抑制Wnt/β-catenin信号通路,是一个潜在的治疗胶质瘤的分子靶点

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