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-  2016 

Wnt信号通路蛋白在宫颈上皮内瘤变及宫颈鳞癌中的表达及其与高危型HPV感染的相关性 Correlation Between the Expression of Wnt Signaling Pathway Protein in Cervical Intraepithelial Neoplasia,Cervical Squamous Cell Carcinoma and High-Risk HPV Infection

Keywords: Wnt信号通路,宫颈上皮内瘤变,宫颈鳞癌,人乳头瘤病毒

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Abstract:

目的:探讨Wnt信号通路蛋白在宫颈上皮内瘤变(CIN)和宫颈鳞癌中的表达及其与高危型HPV感染的相关性。方法:选取119例CIN或宫颈鳞癌患者为研究对象,并随机选取同期因子宫肌瘤行子宫全切术的37例正常宫颈标本作为对照组;利用实时荧光定量PCR和酶联免疫吸附试验分别检测Wnt-11、Frizzled/LRP和ROR2的基因和蛋白表达水平;应用HPV分型检测法测定高危型HPV感染状况。结果:与对照者相比,不同程度宫颈病变患者Wnt-11、LRP-6和ROR2基因和蛋白表达水平均明显升高(P<0.05),且从宫颈炎到宫颈鳞状细胞癌呈现逐渐增加的趋势(P<0.05);FZD-2在宫颈炎、CINⅠ-Ⅲ级的患者中均检测到轻微的增高,但组间比较差异未有统计学意义(P>0.05);共有62例检出高危型HPV,感染率为52.10%,高危型HPV感染率随着宫颈病变级别增高而呈现逐步递增(P<0.05);而且,高危型HPV感染患者的Wnt-11、LRP-6和ROR2表达水平明显高于无高危型HPV感染者(P<0.05);FZD-2的表达量则与是否HPV感染无明显相关(P>0.05)。结论:Wnt-11、LRP-6和ROR2在CIN和宫颈鳞癌中表达增强,且与高危型HPV感染呈正相关

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