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-  2015 

雌激素及其拮抗剂对胃癌细胞系SGC-7901血管内皮细胞生长因子表达的影响 Effect of Estrogen and Its Antagonist on Expression of Vascular Endothelial Growth Factor in Gastric Cancer Cell Line SGC-7901

Keywords: 雌激素,胃肿瘤/治疗应用,血管内皮细胞生长因子,三苯氧胺

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Abstract:

目的:研究雌激素及其拮抗剂对人胃腺癌SGC-7901细胞系血管内皮细胞生长因子(VEGF)表达的影响。方法:用雌二醇及其拮抗剂三苯氧胺(tamoxifen)处理体外培养的胃癌细胞系SGC-7901,在相差显微镜下观察细胞形态的变化;用四甲基偶氮唑蓝(MTT)比色法测定细胞增殖;用双抗体夹心酶联免疫吸附试验(ELISA)和免疫细胞化学SABC法测定细胞培养上清液和细胞质中VEGF蛋白含量。结果:胃癌细胞经雌二醇处理,培养液中VEGF浓度明显高于对照组(P<0.05);而雌二醇加三苯氧胺联合处理胃癌细胞,VEGF浓度有回降,比雌二醇处理组下降。结论:提示雌激素参与调节胃癌细胞VEGF的表达浓度,三苯氧胺有抑制VEGF表达的作用

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