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-  2016 

梓醇对内皮细胞炎症因子表达的影响 Effects of Catalpol on the Inflammatory Factors of Endothelial Cells

Keywords: 梓醇,血管内皮细胞,肿瘤坏死因子-α,白细胞介素-6

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Abstract:

目的:研究梓醇(Catalpol)对高糖高脂诱导的人脐静脉血内皮细胞(HUVECs)炎症反应的影响。方法:体外培养人脐静脉血内皮细胞,用CCK-8法检测不同浓度梓醇溶液对HUVECs细胞增殖活性的影响。将细胞分为空白对照组、高糖高脂组(给予33mmol/L葡萄糖+500μmol/L棕榈酸作用48h)、高糖高脂+1 000mg/L梓醇预处理组(梓醇1 000mg/L预处理24h后,给予33mmol/L葡萄糖+500μmol/L棕榈酸作用48h,以下简称梓醇预处理组),收集细胞上清液,用ELISA检测上清液中的炎症因子TNF-α、IL-6的浓度。结果:梓醇作用于HUVECs细胞的最佳浓度为1 000mg/L。高糖高脂组中TNF-α和IL-6浓度较空白对照组均明显增高(t=7.03、6.68,P均<0.01)。与高糖高脂组比较,梓醇预处理组上清液中炎症因子TNF-α及IL-6浓度均下降(t=-2.87、-3.76,P均<0.05)。结论:高糖高脂可诱导血管内皮细胞炎症反应,而梓醇对高糖高脂诱导的炎症反应有一定的改善作用

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