全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...
-  2017 

白细胞介素-33上调A549细胞中黏蛋白基因MUC5AC和MUC5B的表达 Interleukin-33 Promotes the Expression of MUC5AC and MUC5B in A549 Cells

Keywords: 白细胞介素-33,黏蛋白,MUC5AC,NF-κB信号通路

Full-Text   Cite this paper   Add to My Lib

Abstract:

目的:探讨白细胞介素-33(IL-33)对A549细胞中黏蛋白基因表达的影响及其作用机制。方法:体外培养人肺腺癌细胞A549,给予IL-33刺激,通过荧光定量PCR技术(RT-PCR)检测黏蛋白家族基因MUC1,MUC5AC,MUC5B和NF-κB下游基因的mRNA水平,Western Blot检测IκBα的磷酸化水平。随后在IL-33刺激的A549细胞中加入NF-κB抑制剂PDTC,将A549细胞分为对照组,IL-33刺激组,PDTC处理组以及IL-33和PDTC共同处理组,RT-PCR检测黏蛋白家族基因MUC5AC和MUC5B的mRNA水平。结果:IL-33(100ng/ml)处理24h后,A549细胞中MUC5AC和MUC5B的mRNA水平高于对照组(P<0.05),同时NF-κB下游基因(RELA,RELB,NFκB2)的表达也显著高于对照组(P<0.01)。IL33处理A549细胞5min后,IκBα的磷酸化水平显著高于对照组。随后加入NF-κB通路抑制剂PDTC可以有效抑制IL33对MUC5AC基因的激活(P<0.05),而PDTC对MUC5B基因的激活没有影响。结论:IL-33可以通过激活NF-κB信号通路来上调A549细胞中MUC5AC基因的表达,而IL-33上调MUC5B的表达则可能是通过其他信号通路

References

[1]  Oue N,Sentani K,Sakamoto N,et al.Clinicopathologic and molecular characteristics of gastric cancer showing gastric and intestinal mucin phenotype[J].Cancer Science,2015,106(8):951-958.
[2]  Ma R,Wang Y,Cheng G,et al.MUC5AC expression up-regulation goblet cell hyperplasia in the airway of patients with chronic obstructive pulmonary disease[J].Chinese Medical Sciences Journal,2005,20(3):181-184.
[3]  Ovrevik J,Refsnes M,Lag M,et al.Activation of Proinflammatory Responses in Cells of the Airway Mucosa by Particulate Matter:Oxidant and Non-Oxidant-Mediated Triggering Mechanisms[J].Biomolecules,2015,5(3):1 399-1 440.
[4]  Van Bruaene N,Bachert C.Tissue remodeling in chronic rhinosinusitis[J].Current Opinion in Allergy and Clinical Immunology,2011,11(1):8-11.
[5]  Kufe DW.Mucins in cancer:function,prognosis and therapy[J].Nature Reviews Cancer,2009,9(12):874-885.
[6]  Hugen N,Simons M,Halilovic A,et al.The molecular background of mucinous carcinoma beyond MUC2[J].J Pathol Clin Res,2015,1(1):3-17.
[7]  Bae CH,Kim JS,Song SY,et al.Insulin-like growth factor-1induces MUC8 and MUC5B expression via ERK1and p38 MAPK in human airway epithelial cells[J].Biochem Biophys Res Commun,2013,430(2):683-688.
[8]  Shang J,Zhao J,Wu X,et al.Interleukin-33promotes inflammatory cytokine production in chronic airway inflammation[J].Biochem Cell Biol,2015,93(4):359-366.
[9]  Lu J,Kang J,Zhang C,et al.The role of IL-33/ST2L signals in the immune cells[J].Immunology Letters,2015,164(1):11-17.
[10]  Sheng YH,Hasnain SZ,Florin TH,et al.Mucins in inflammatory bowel diseases and colorectal cancer[J].J Gastroenterol Hepatol,2012,27(1):28-38.
[11]  Oboki K,Nakae S,Matsumoto K,et al.IL-33and Airway Inflammation[J].Allergy,Asthma&Immunology Research,2011,3(2):81-88.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133