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-  2017 

红景天苷保护慢性间断性缺氧心肌细胞作用及机制 Protective Effect of Salidroside on Myocardial Cells of Chronic Intermittent Hypoxia and Its the Mechanism

Keywords: 红景天苷,凋亡,慢性间断性缺氧,Bcl-2,Bax,Caspase-3

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Abstract:

目的:探讨红景天苷对慢性间断性缺氧心肌细胞的影响及作用机制。方法:建立大鼠慢性间断性缺氧模型,红景天苷进行干预,实验分为假手术组、NaCl组及红景天苷组。检测心脏功能,TUNEL法检测心肌组织的心肌细胞凋亡情况。Western Blot检测心肌组织Bcl-2、Bax、caspase-3的表达情况。同时测定心肌组织caspase-3的活性。结果:与对照组比较,红景天苷干预组8周,心功能明显改善,心肌细胞的凋亡明显减少(P<0.05)。同时红景天苷干预组Bcl-2和Bax的表达比例更趋于正常化,caspase-3的表达及活性则明显减少(P<0.05)。结论:在慢性间断性缺氧中,红景天苷通过平衡Bcl-2和Bax的表达,抑制caspase-3的活性而对抗心肌细胞的凋亡,保护心功能

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