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-  2015 

草果挥发油对肝癌H_(22)荷瘤小鼠的抑瘤作用 Anti-Tumor Effect of the Essential Oil from Amomum tsao-ko on H_(22) Tumor Bearing Mice

Keywords: 草果挥发油,肝癌H22细胞,抗肿瘤,细胞凋亡,Bax蛋白,Bcl-2蛋白

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Abstract:

建立肝癌H22荷瘤小鼠模型,探讨了草果挥发油对肿瘤质量、胸腺指数和脾脏指数的影响,采用HE染色法观察肿瘤细胞形态变化,免疫组化法检测肿瘤细胞凋亡相关蛋白Bax和Bcl-2的表达.结果发现,草果挥发油各剂量组均能明显抑制肿瘤生长(p<0.05);高剂量组(300 mg·kg-1)有些微毒副作用,各剂量组胸腺指数和脾脏指数较环磷酰胺(CTX)组有明显升高(p<0.05);推测草果挥发油能够抑制H22荷瘤小鼠肿瘤细胞生长的机制是通过上调Bax蛋白表达,下调Bcl-2蛋白表达来诱导细胞凋亡

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