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- 2016
野樱莓多酚提取剂对多酚抗氧化活性和可浓缩性的影响
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Abstract:
摘要： 多酚所具有的保健作用逐渐被人们所认识,含有丰富多酚类化合物的野樱莓(Aronia melanocarpa)也逐渐受到关注。高效提取野樱莓中总多酚是其高值化利用的重要途径。本文对比研究了利用酸性乙醇、乙醇和纯水3种提取剂对野樱莓中总多酚的提取效果。结果表明,酸性乙醇具有最高的多酚提取率,而以纯水作为提取剂其多酚提取量只有酸性乙醇的28.77%。对多酚抗氧化活性(去除DPPH自由基的能力)研究发现,水提多酚的抗氧化活性高于乙醇和酸性乙醇提取的多酚。酸性乙醇、乙醇和纯水提取的多酚去除DPPH自由基的VC当量比值为100∶76.10∶70.83。将3种提取液减压浓缩至原体积的10%后,多酚的抗氧化活性几乎不损失,但是3种浓缩液在冷冻干燥后,只有水提多酚可以完全复溶。综合考虑成本、安全等因素,纯水作为野樱莓多酚的提取剂具有一定优势。
Abstract: Since the healthy effects of polyphenols have been gradually recognized, Aronia melanocarpa, a polyphenols-rich berry, received more and more attentions. Efficient extraction of total polyphenols from this kind of berry was an important way of the high-value usage. In this paper, a comparative study of extracting effects of acidic ethanol, ethanol and water on Aronia melanocarpa polyphenols was performed. The results showed that acidic ethanol had the highest polyphenols extraction ratio, while the extraction ratio of water was 28.77% of acidic ethanol. The analysis of polyphenols antioxidant activity(DPPH radical removing capability)showed that the water-extracted polyphenols had a higher antioxidant activity than those extracted from alcohol and acidic ethanol. The ratio of total DPPH radical removal capability of acidic ethanol-, ethanol- and water-extracted polyphenols was 100∶76.10∶70.83. In addition, the condensabilities of the three kinds of extractions were compared. When the extractions were evaporated to 10% of original volume, the antioxidant activity was almost not lost. However, after being freeze-dried, only the water-extractd polyphenol was 山 东 大 学 学 报 (理 学 版)第51卷 - 第5期李曌,等:野樱莓多酚提取剂对多酚抗氧化活性和可浓缩性的影响 \=-completely reconstituted. Considering the cost, safety and other factors, water had certain advantages to Aronia melanocarpa polyphenols extraction
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