环境因素对3株嗜盐聚磷菌除磷性能的影响
Environmental factors influence on phosphorus removal of three halophilic phosphate accumulating bacteria strains

摘要： 从运行稳定的以生活污水为碳源的污泥中富集分离,并筛选出3株嗜盐聚磷菌菌株:qdp01、菌株qdp03和菌株qdp05。通过对菌株的形态、生理生化特征及16S rDNA序列进行分析后,确定菌株qdp01属于产碱杆菌属(Alcaligenes sp.);菌株qdp03和菌株qdp05为肠杆菌属(Enterobacter sp.)。通过改变温度、pH、碳源、盐度等环境因素,研究以上因素对3株菌除磷效果的影响,得出了3株菌除磷的最佳环境条件。当温度为30 ℃、pH值在7.5～9.0、碳源为乙酸钠、盐度为2%时,3株菌对磷的去除率均可达到80%以上。特别研究了不同盐度对菌株生长和除磷性能的影响,结果显示3株菌在高盐环境中磷的去除有较高的应用价值。
Abstract: Three halophilic phosphate accumulating bacterial strains qdp01, qdp03, qdp05 were separated and screened from the biological phosphorus removal sludge which was supplied with domestic sewage as carbon source under steady operation. The strain qdp01 were identified as Alcaligenes sp. by analyzing the morphology, physiology, biochemical features and 16S rDNA sequence. The strain qdp03 and qdp05 was identified as Enterobacter sp. Through researching their phosphorus removal by changing the temperature, pH, carbon sources and salinity, we get best environmental conditions for phosphorus removal. The results were as follows: when sodium acetate was used as carbon sources, temperature 30 ℃, pH 7.5-9, salinity 2%, the removal rates of the PO43- by the three strains were all over 80%. We especially researched the effect of different salinity on strains growth and phosphorus removal, so that the three strains have great application value for phosphorus removal of hyperhaline wastewater