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- 2015
光谱法研究nC60纳米颗粒与牛血红蛋白的相互作用
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Abstract:
摘要： 用几种光谱方法研究了nC60纳米颗粒与牛血红蛋白之间的相互作用。用溶剂置换法制备nC60的水分散液,并用紫外-可见分光光度法、动态光散射和透射电子显微镜技术对nC60纳米颗粒进行表征;用紫外-可见分光光谱、荧光光谱、同步荧光光谱研究nC60与牛血红蛋白之间的作用。结果表明,nC60纳米颗粒对牛血红蛋白的紫外-可见吸收光谱有一定程度的改变作用;nC60可猝灭牛血红蛋白的内源荧光,且最大发射波长发生明显的蓝移现象;随着nC60浓度的增加,酪氨酸残基和色氨酸残基的同步荧光强度降低,其中酪氨酸残基峰位发生蓝移,而色氨酸残基峰位基本保持不变。研究表明nC60与牛血红蛋白之间存在一定的相互作用;nC60可引起牛血红蛋白构象的改变,酪氨酸残基所处微环境的极性减小,nC60与牛血红蛋白的作用位置更接近酪氨酸残基。
Abstract: Several spectroscopic methods were used to study the interaction between nC60 nanoparticles and bovine hemoglobin. The nC60 nanoparticles dispersion in water was prepared by solvent replacement method and characterized using UV-Vis spectrometry, dynamic light scattering and transmission electron microscopy. The interaction between nC60 and bovine hemoglobin was investigated by UV-Vis spectrometry, fluorescence spectroscopy and synchronous fluorescence spectroscopy. The result indicated that the UV-Visible absorption spectrum of bovine hemoglobin was changed to some extent by nC60 nanoparticles. The intrinsic fluorescence of bovine hemoglobin could be quenched by nC60 and the maximum emission wavelength showed obvious blue-shift. With the increasing concentrations of nC60, the synchronous fluorescence intensity of tyrosine and tryptophan residues decreased gradually, and the fluorescence peak of tyrosine residues exhibited a blue shift while the fluorescence peak of tryptophan residues remained unchanged. The research showed that the binding of nC60to bovine hemoglobin could cause the conformation change of bovine hemoglobin. The microenvironment polarity around tyrosine residues decreased, indicating the binding site of nC60 to bovine hemoglobin was located near tyrosine residues
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