全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...
-  2018 

基于铁氰化钾电子媒介体及醛基吡啶盐的电化学免疫传感器
A Label-free Immunosensor Based on?K3[Fe(CN)6] and Aldehyde-Functionalized Pyridinium

DOI: 10.13208/j.electrochem.170406

Keywords: 电化学免疫传感器,K3[Fe(CN)6],醛基吡啶盐,甲胎蛋白,
electrochemical immunosensor
,K3[Fe(CN)6],aldehyde-functionalized pyridinium,α-Fetoprotein

Full-Text   Cite this paper   Add to My Lib

Abstract:

摘要 采用聚二烯丙基二甲基氯化铵(PDDA)将铁氰化钾电子媒介体固定在电极表面,构建免标记的电化学免疫传感器. 醛基吡啶盐不仅作为基底物质直接固定抗体,还可以很好地增强电极表面的导电性能. 将构建的传感器用于肿瘤标志物甲胎蛋白的检测. 其线性范围为0.01-20 ng·mL-1,检测下限为0.004 ng·mL-1(3 S/N). 此传感器的构建简单方便、无标记、特异性好,为甲胎蛋白及其他肿瘤标志物提供了新的检测方法

References

[1]  Weng S H, Chen M, Zhao C F, et al. Label-free electrochemical immunosensor based on K3[Fe(CN)6] as signal for facile and sensitive determination of tumor necrosis factor-alpha[J]. Sensors and Actuators B, 2013, 184: 1-7.
[2]  Muthirulan P, Velmurugan R. Direct electrochemistry and electrocatalysis of reduced glutathione on CNFs-PDDA/PB nanocomposite film modified ITO electrode for biosensors[J]. Colloids and Surfaces B, 2011, 83(2): 347-354.
[3]  Li R, Wu K B, Liu C X, et al. 4-Amino-1-(3-mercapto-propyl)-pyridine hexa?uorophosphate ionic liquid functionalized gold nanoparticles for IgG immunosensing enhancement[J]. Analytical Chemistry, 2014, 86(11): 5300-5307.
[4]  Lin J H, Wei Z J, Chu P F. A label-free immunosensor by controlled fabrication of monoclonal antibodies and gold nanoparticles inside the mesopores[J]. Analytical Biochemistry, 2012, 421(1): 97-102.
[5]  Zhang J, He J L, Xu W L, et al. A novel immunosensor for detection ofμ-galactosideβ-2, 6-sialyltransferase in serum based on gold nanoparticles loaded on Prussian blue-based hybrid nanocomposite film[J]. Electrochimica Acta, 2015, 156: 45-52.
[6]  Lai WQ, Tang D P, Que X H, et al. Enzyme-catalyzed silver deposition on irregular-shaped gold nanoparticles for electrochemical immunoassay of alpha-fetoprotein[J]. Analytica Chimica Acta, 2012, 755: 62-68.
[7]  Qiu J D, Liang R P, Wang R, et al. A label-free amperometric immunosensor based on biocompatible conductive redox chitosan-ferrocene/gold nanoparticles matrix[J]. Biosensenors and Bioelectronics, 2009, 25(4): 852-857.
[8]  Jiao S F, Jin J, Wang L. One-pot preparation of Au-RGO/PDDA nanocomposites and their application for nitrite sensing[J]. Sensors and Actuators B, 2015, 208(2): 36-42.
[9]  Amatatongchai M, Sroysee W, Chairam S, et al. Simple flow injection for determination of sulfite by amperometric detection using glassy carbon electrode modified with carbon nanotubes-PDDA-gold nanoparticles[J]. Talanta, 2015, 133: 134-141.
[10]  Plater M J, Barnes P, McDonald L K, et al. Hidden signatures: new reagents for developing latent fingerprints[J]. Organic and Biomolecular Chemistry, 2009, 7(8): 1633-1641.
[11]  Wang X, Zhang Q Y, Li Z J, et al. Development of high-performance magnetic chemiluminescence enzyme immunoassay for α-fetoprotein (AFP) in human serum[J]. Clinica Chimica Acta, 2008, 393(2): 90-94.
[12]  Shen G, Hu X, Zhang S. A signal-enhanced electrochemical immunosensor based on dendrimer functionalized-graphene as a label for the detection ofμ-1-fetoprotein[J]. Journal of Electroanalytical Chemistry, 2014, 717: 172-176.
[13]  Lin J, Wei Z, Zhang H, et al. Sensitive immunosensor for the label-free determination of tumor marker based on carbon nanotubes/mesoporous silica and graphene modified electrode[J]. Biosensors and Bioelectronics, 2013, 41: 342-347.
[14]  Feng T, Qiao X, Wang H, et al. A sandwich-type electrochemical immunosensor for carcinoembryonic antigen based on signal amplification strategy of optimized ferrocene functionalized Fe3O4@SiO2 as labels[J]. Biosensors and Bioelectronics 2016, 79(4): 48-54.
[15]  Shi W T, Ma Z F. A novel label-free amperometric immunosensor for carcinoembryonic antigen based on redox membrane[J], Biosensors and Bioelectronics. 2011, 26(6): 3068-3071.
[16]  Liu D, Wu F, Zhou C, et al. Multiplexed immunoassay biosensor for the detection of serum biomarkers-HCG and AFP of Down Syndrome based on photoluminescent water-soluble CdSe/ZnS quantum dots[J]. Sensors and Actuators B, 2013, 186: 235-243.
[17]  Lin X(林晓), Weng S H(翁少煌)袁Zhou J Z(周剑章),et al. Electrochemical Immunosensor based on polycalconcarboxylic acid modified electrode for the determination of alpha-fetoprotein[J]. Journal of Electrochemistry(电化学), 2012,18(4):371-376.
[18]  Yin Z Z, Liu Y, Jiang L P, et al. Electrochemical immunosensor of tumor necrosis factor α based on alkaline phosphatase functionalized nanospheres[J]. Biosensors and Bioelectronics, 2011, 26(5): 1890-1894.
[19]  Han J M, Ma J, Ma Z F. One-step synthesis of graphene oxide-thionine-Au nanocomposites and its application for electrochemical immunosensing[J]. Biosensors and Bioelectronics, 2013, 47(10): 243-247.
[20]  Kavosi B, Salimi A, Hallaj R, et al. A highly sensitive prostate-specific antigen immunosensor based on gold nanoparticles/PAMAM dendrimer loaded on MWCNTS/chitosan/ionic liquid nanocomposite[J]. Biosensors and Bioelectronics, 2014, 52: 20-28.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133