Cd 2+处理后麻雀脑组织中糖皮质激素受体和盐皮质激素受体表达变化
Expression of Glucocorticoid Receptors and Mineralocorticoid Receptors in the Brain of Passer montanus after Cadmium Treatment

中文摘要： 目的 检测在Cd 2+处理后糖皮质激素受体(GR)和盐皮质激素受体(MR)在麻雀 Passer montanus脑组织中的表达变化。 方法 6只麻雀随机分成2组,每组3只。处理组饮水中添加500μg·L -1 Cd 2+,对照组不添加。用ClustalW2将麻雀的GR和MR氨基酸序列与其他物种氨基酸序列进行相似性比对分析。采用Real-time PCR检测基础水平下麻雀各组织中GR和MR基因的表达水平。Cd 2+处理后,测定GR和MR基因在脑组织中的表达变化。 结果 麻雀与斑胸草雀 Taeniopygia guttata和原鸡 Gallus gallus的GR和MR氨基酸序列的相似性大于90%;GR和MR基因在麻雀肝脏、肠、心脏、眼球、脑、肺、肌肉、肾脏和性腺9个组织中均有表达;Cd 2+处理后,处理组脑组织中GR和MR mRNA表达水平均上调,且显著高于对照组( P<0.05)。 结论 GR和MR在麻雀各组织中广泛表达;推测GR和MR基因在Cd 2+的应激反应中具有重要作用,具体作用还有待于进一步论证,同时该研究为野外工作提供了一定的实验依据。
英文摘要： Objective The expression levels of glucocorticoid receptors (GR) and mineralocorticoid receptors (MR) were measured in the brain of Passer montanus after cadmium treatment. Method Six P. montanus were randomly divided into 2 groups. Treatment group was fed with water of 500μg·L -1 Cd 2+. Alignment of amino acid sequence of GR and MR was performed using the ClustalW2. Real-time PCR was performed to study the baseline expression levels of GR and MR among different tissues. Finally, the expressions of GR and MR were determined in the brain of P. montanus after cadmium treatment. Result The result of amino acid sequence alignment showed that the GR and MR of P. montanus had high similarity with Taeniopygia guttata and Gallus gallus (>90%). Real-time PCR results showed that the GR and MR expressed in all of nine detected organs and tissues. Compared with the control, the expression levels of GR and MR were significantly up-regulated after cadmium treatment ( P<0.05). Conclusion The GR and MR were widely expressed in tissues of P. montanus. GR and MR might play a significant role under the cadmium stress. This research provided the experimental evidence for fieldwork. 2016,(35): 409-413 收稿日期：2015-11-05 DOI：10.11984/j.issn.1000-7083.20150346 分类号：Q786;Q959.7 基金项目：陕西省科学院重大项目(2013K-01);国家自然科学基金项目(31372201) 作者简介：索丽娟(1985-),女,硕士,研究实习员,研究方向:秦岭水源涵养区水质污染对鸟类毒性效应,E-mail:slj1030@163.com *通讯作者：赵洪峰,男,副教授,E-mail:zhaohf@snnu.edu.cn 参考文献： 邓琼. 2014. 垂体促肾上腺皮质激素细胞的糖皮质激素快速反馈机制研究[D]. 长春:吉林大学. 张丹, 张军, 欧阳盼, 等. 2013. 南昌市常见鸟类对环境中Cu、Pb、Cd重金属污染物的指示作用研究[J]. 江西师范大学学报(自然科学版), 37(3):319-323. Aguilera G. 1998. Corticotrophin releasing hormone, receptor regulation and the stress response[J]. Trends in Endocrinology & Metabolism, 9(8):329-336. Burger J, Eichhorst B. 2005. Heavy metals and selenium in grebe eggs from Agassiz National Wildlife Refuge in northern Minnesota[J]. Environmental Monitoring and Assessment, 107(1-3):285-295. Burger J, Eichhorst B. 2007. Heavy metals and selenium in grebe feathers from Agassiz National Wildlife Refuge in northern Minnesota[J]. Archives of Environmental