日本三角涡虫成体干细胞的异质性研究进展
The Heterogeneity of Neoblasts in the Planarian Dugesia japonica

中文摘要：Neoblasts是日本三角涡虫 Dugesia japonica成体体内唯一具有增殖和分化能力的多能干细胞，是其损伤修复和再生的细胞基础。Neoblasts的组成是均质还是异质，长期以来一直存在争议。近年来研究表明，neoblasts具有异质性，由不同的细胞亚群组成。本文从形态、射线抗性、细胞周期及分子标志等4个方面综述了neoblasts异质性研究的最新进展，为将来探讨neoblasts不同亚群功能、相互关系及各亚群在涡虫再生过程中的作用提供借鉴。
英文摘要：Neoblasts are considered as the sole somatic stem cells in the planarian Dugesia japonica with the capacity to proliferate and give rise to all cell types during regeneration, and they are the cellular basis for wound repair and regeneration. Previous researches demonstrated that neoblasts were composed by different subpopulations, which meant that they were heterogeneous. We reviewed the heterogeneity of neoblasts in aspects of mophology, radio-resistance, cell cycle and molecular markers, which was important for further studying the relationships between neoblast subpopulations, their effects on planarian regeneration and understanding the survival strategy of planarians. 2016,35(5): 797-800 收稿日期：2016-04-01 DOI：10.11984/j.issn.1000-7083.20160070 分类号：Q959.151;Q952 基金项目：国家自然科学基金项目（31471965）；河南省教育厅河南省高等学校重点科研项目（15A180017）；河南省人力资源和社会保障厅河南省博士后科研项目（2012059）；河南师范大学国家级科研项目培育基金项目（2012PL06）；河南省基础与前沿研究计划项目（162300410148） 作者简介：宇文延青(1970-),男,副教授,主要从事动物资源保护与利用方面的研究,E-mail:ywyanqing@163.com *通讯作者：陈广文,男,博士,教授,主要从事中国淡水涡虫分类学研究,E-mail:chengw0183@sina.com 参考文献： Baguna J, Romero R, Saló E, et al. 1990. Growth, degrowth and regeneration as developmental phenomena in adult freshwater planarians[C]//Marthy HJ. Experimental embryology in aquatic plants and animals. New York: Springer US: 129-162. Coward SJ. 1974. Chromatoid bodies in somatic cells of the planarian: observations on their behavior during mitosis[J]. The Anatomical Record, 180(3): 533-545. Eisenhoffer GT, Kang H, Alvarado AS. 2008. Molecular analysis of stem cells and their descendants during cell turnover and regeneration in the planarian Schmidtea mediterranea[J]. Cell Stem Cell, 3(3): 327-339. Guo T, Peters AH, Newmark PA. 2006. A Bruno-like gene is required for stem cell maintenance in planarians[J]. Developmental Cell, 11(2): 159-169. Hayashi T, Asami M, Higuchi S, et al. 2006. Isolation of planarian X-ray-sensitive stem cells by fluorescence-activated cell sorting[J]. Development, Growth & Differentiation, 48(6): 371-380. Hayashi T, Shibata N, Okumura R, et al. 2010. Single-cell gene profiling of planarian stem cells using fluorescent activated cell sorting and its "index sorting" function for stem cell research[J]. Development, Growth & Differentiation, 52(1): 131-144. Higuchi S, Hayashi T, Hori I, et al. 2007. Characterization and categorization of fluorescence