牦牛肠源 Lactobacillus acidophilus L3对其小肠黏膜形态结构的影响
Effect of Intestinal Lactobacillus acidophilus L3 on the Morphological Structure of Small Intestine in Yaks

中文摘要：为了探讨牦牛肠源嗜酸乳杆菌 Lactobacillus acidophilus L3对牦牛小肠肠道黏膜形态结构的影响,将10头健康牦牛(2~2.5岁)随机分为2组,分别为益生菌组和空白对照组,益生菌组在饲料中添加2×10 9 CFU·kg -1 L. acidophilus L3,饲喂28 d后取其小肠组织样品,采用组织学和显微测量学方法对益生菌组和空白对照组牦牛小肠绒毛高度、隐窝深度、绒毛高度/隐窝深度比值(V/C),以及回肠黏膜淋巴集结厚度进行测量和统计分析。研究结果显示,益生菌组牦牛十二指肠、空肠、回肠绒毛高度均较空白对照组显著增加,隐窝深度降低,V/C值增加,差异有统计学意义( P<0.05)。通过对回肠淋巴集结的测量发现,益生菌组牦牛回肠淋巴集结的平均厚度均较空白对照组显著增加,差异有统计学意义( P<0.05)。研究结果表明,牦牛肠源 L. acidophilus L3可有效改善其肠道黏膜结构,增强肠道黏膜免疫效应部位的免疫功能。
英文摘要：Species diversity of fish was investigated in the upper streams of Lijiang River (including four tributaries, such as Xiaorong River, Chuan River, Ludong River and Huangbai River) from April 2013 to January 2014. A total of 72 freshwater fish species belo 2015,(): 76-80 收稿日期：2014-4-10 DOI：10.3969/j.issn.1000-7083.2015.01.012 分类号：S85 基金项目：陕西省教育厅科学研究计划(自然科学专项)项目2013JK0721;陕西理工学院人才启动项目SLGQD13-19;农业部兽用药物创制重点实验室和甘肃省新兽药工程重点实验室开放课题(1610322011011) 作者简介：祁珊珊(1984-),女,博士,讲师,主要从事动物病理学研究,E-mail:qishanshan101@126.com *通讯作者：郑红星,博士,讲师,主要从事药理毒理学研究,E-mail:zhenghongxing100@126.com 参考文献： 郭元晟, 闫素梅, 史彬林, 等. 2011. 发酵乳酸杆菌对肉鸡小肠绒毛形态的影响[J]. 动物营养学报, 23(7): 1194-1200. 乔宏宇, 郎仲武, 董克苏, 等. 1994. 接续产酸型活菌制剂对仔猪生产性能的影响及机理初探[J]. 吉林农业大学学报, 16(2): 74-80. 王长文, 李永近, 栾维民, 等. 1998. 接续产酸型活菌制剂对犊牛小肠绒毛形态的影响[J]. 吉林农业大学学报, 20(4): 68-70. 杨淑静. 2001. 黏膜免疫[J]. 国外医学: 免疫学分册, 24(6): 296-299. Awad WA, Ghareeb K, Abdel R, et al. 2009. Effects of dietary inclusion of probiotic and synbiotic on growth performance, organ weights, and intestinal histomorphology of broiler chickens[J]. Poult Sci, 88(1): 49-56. Fagarasan S, Honjo T. 2003. Intestinal IgA synthesis: regulation of front line body defences[J]. Nat Rev Immunol, 3(1): 63-72. Macpherson AJ, Geuking MB, McCoy KD. 2005. Immune responses that adapt the intestinal mucosa to commensal intestinal bacteria[J]. Immunology, 115(2): 153-162. Mowat AM. 2003. Anatomical basis of tolerance and immunity to intestinal antigens[J]. Nat Rev Immunol, 3: 331-341. Samli HE, Senkoylu N, Koc F, et al. 2007. Effects of Enterococcus faecium and dried whey on broiler performance, gut histomorphology and intestinal microbiota[J]. Arch Anim Nutr, 61(1): 42-49. Tufano MA, Cipollaro LG, Innielo R, et al. 1991. Protein A and other surface components of Staphylococcus aureus stimulate production of IL-1, IL-6, TNF and IFN-γ[J]. Eur Cytokine Netw, 2: 361-366. Willing BP, Van Kessel AG. 2007. Enterocyte proliferation and apoptosis in the caudal small intestine is influenced by the composition of colonizing commensal bacteria in the neonatal gnotobiotic pig[J]. Journal of Animal Science, 85(12): 3256-3266. Yang H, Liu A, Zhang M, et al. 2009.