家蚕昼夜节律生物钟基因的生物信息学分析
Bioinformatics Analysis of Circadian Rhythm Biological Clock Genes in Bombyx mori

中文摘要：昼夜节律是最普遍的生物节律现象,受遗传基因调控,其分子机制在黑腹果蝇 Drosophila melanogaster中有较为深入的研究,在其他昆虫中的研究相对较少。家蚕 Bombyx mori的滞育是对昼夜节律授时因子响应的一种现象,可作为研究的参照。通过电子克隆的方法获得了家蚕生物钟基因 Bmvri, Bmcyc, Bmtim2, Bmpdp完整的开放阅读框(ORF)序列,以及 Bmclk基因的ORF片段,并对上述基因及其表达产物进行了结构分析、染色体定位和系统的分子进化分析,根据这些基因及其表达产物的结构特征结合现有的数据资源,整合了家蚕昼夜节律生物钟反馈环路。
英文摘要：Circadian rhythm is the most common phenomenon of biological rhythms, which is regulated by the genetic clock genes. Intensive research about molecular mechanism of circadian rhythm has been carried out in Drosophila melanogaster. Diapause of the silkworm ( Bombyx mori) is a phenomenon to response the circadian rhythm zeitgeber, therefore it can be used as reference to circadian rhythm research. The open reading frame (ORF) sequences of Bmvri, Bmcyc, Bmtim2, Bmpdp and Bmclk fragment were cloned in silico, followed by genetic structure analysis, chromosome location and molecular phylogenetic analysis of those genes and their expression products. With the data resources, the current feedback loop of circadian rhythm in silkworm was integrated according to the structural characteristics of biological clock genes and their expression products. 2016,(35): 275-282 收稿日期：2015-10-13 DOI：10.11984/j.issn.1000-7083.20150316 分类号：S8;Q78 基金项目：国家自然科学基金项目"基于 Ea4与Sod基因互作的滞育生物钟分子调控机制研究"(30771632); 国家自然科学基金项目"基于家蚕昼夜节律生物钟信号途径基因发掘的滞育诱导授时信号输入机制研究"(31172264) 作者简介：王文栋, 女, 硕士, 讲师, 研究方向:生物化学与分子生物学、生物信息学, E-mail:190594526@qq.com *通讯作者：徐世清, 博士, 教授, 研究方向:发育功能基因与分子调控机理、动物分子遗传与基因工程, E-mail:xushiqingsd@126.com 参考文献： 郭安源, 朱其慧, 陈新, 等. 2007. GSDS: 基因结构显示系统[J]. 遗传, 29(8): 1023-1026. 王文栋, 梁辉, 朱晓苏, 等. 2011. 家蚕生物钟基因 Bmcry 1与 Bmcry 2的克隆及生物信息学分析[J]. 昆虫学报, 54(1): 9-19. 王文栋. 2011. 家蚕TIME-EA4进化及昼夜节律生物钟基因的克隆、进化和反馈环路研究[D]. 苏州: 苏州大学: 80-89. Codd V, Dolezel D, Stehlik J, et al . 2007. Circadian rhythm gene regulation in the housefly Musca domestica [J]. Genetics, 177: 1539-1551. Crooks GE, Hon G, Chandonia JM, et al . 2004. WebLogo: a sequence logo generator[J]. Genome Research, 14: 1188-1190. Florea L, Hartzell G, Zhang Z, et al . 1998. A computer program for aligning a cDNA sequence with a genomic DNA sequence[J]. Genome Research, 8(9): 967-974. Gasteiger E, Gattiker A, Hoogland C, et al . 2003. ExPASy: the proteomics server for in-depth protein knowledge and analysis[J]. Nucleic Acids Research, 31(13): 3784-3788. Giebultowicz JM. 2001. Peripheral clocks and their role in circadian timing: insights from insects[J]. Philosophical Transactions of the Royal Society B: Biological Sciences, 356(1415): 1791-1799. Hall JC. 2003. Genetics and molecular biology of rhythms in Drosophila and other insects[J].