川芎定痛颗粒对硝酸甘油致偏头痛大鼠作用的研究
Study of the Effect of the Chuanxiong Dingtong Grains on the Rats with Migraine

中文摘要： 目的 观察川芎定痛颗粒对硝酸甘油致偏头痛大鼠的作用。 方法 将60只SD大鼠随机分为正常对照组、模型组、佐米曲普坦组(0.83 mg每千克体质量)、川芎定痛颗粒高剂量组(1.75 g浸膏粉每千克体质量)、中剂量组(0.88 g浸膏粉每千克体质量)、低剂量组(0.44 g浸膏粉每千克体质量)6组,灌胃给予相应药物5 d后,除正常对照组外,对大鼠皮下注射硝酸甘油每千克体质量10 mg,建立偏头痛大鼠模型,通过ELASA方法检测大鼠血浆中5-羟色胺(5-HT)、内皮素(ET)、去甲肾上腺素(NE)和脑组织中5-HT、多巴胺(DA)、NE的含量。 结果 与正常对照组比较,模型组血浆中5-HT与ET含量明显降低,差异有统计学意义( P<0.05),川芎定痛颗粒高、中剂量组可明显升高其含量,与模型组比较差异有统计学意义( P<0.05),佐米曲普坦组、川芎定痛颗粒低剂量组有升高的趋势;NE含量明显降低,正常对照组与模型组比较差异有统计学意义( P<0.05),川芎定痛颗粒高剂量组可明显升高其含量,而佐米曲普坦组、川芎定痛颗粒中、低剂量组则是含量降低,与模型组比较差异均有统计学意义( P<0.05)。与正常对照组比较,模型组脑组织内5-HT含量差异虽无统计学意义,但降低趋势明显,且川芎定痛颗粒高剂量组能明显升高其含量,与模型组比较有统计学意义( P<0.05),佐米曲普坦组、中、低剂量亦有升高的趋势;与正常对照组比较,模型组脑组织内NE含量明显升高,有统计学差异( P<0.05),川芎定痛颗粒高、中剂量组能明显降低其含量,与模型组比较有统计学差异( P<0.05);与正常对照组比较,模型组脑组织中DA含量降低,有统计学差异( P<0.05),川芎定痛颗粒高、中剂量组能升高其含量,具有统计学意义( P<0.05)。 结论 川芎定痛颗粒对硝酸甘油致偏头痛大鼠有治疗作用,其机制可能与调节模型大鼠血浆及脑组织内单胺类神经递质5-HT、NE、ET、DA含量有关。
英文摘要： Objective To investigate the effect of Chuanxiong Dingtong grains (CXDT grains) on the rats with migraine. Method A total of 60 rats were randomly divided into 6 groups including the normal control group, the model control group, the zolmitriptan control group (0.83 mg·kg -1), the high-dose (1.75 g powdered extract per kg), the medium-dose (0.88 g powdered extract per kg) and the low-dose group (0.44 g powdered extract per kg). Five days after intragastric administration of appropriate drugs, except the normal control group, the rats were subcutaneously injected with nitroglycerin (10 mg·kg -1) to establish migraine rat model. The content of 5-hydroxytryptamine (5-HT), norepinephrine (NE), endothelin (ET) in the plasma and 5-HT, NE, dopamine (DA) in the brain were tested by ELASA. Results Compared with the normal control group, the contents of 5-HT, NE, and ET were significantly decreased ( P<0.05) in the plasma of the migraine model rats, and the content of 5-HT in brain tissue showed a decreasing trend, the content of NE was significantly increased ( P<0.05), and the content of DA was significantly decreased ( P<0.05). Therefore, CXDT grains can significantly improve the active substance in rat plasma and brain neurotransmitter content of migraine rat model. Conclusion CXDT grains had a positive effect on the rats with migraine by adjusting the levels of the brain neurotransmitters. 2016,(35): 252-255 收稿日期：2015-11-18 DOI：10.11984/j.issn.1000-7083.20150364 分类号：Q95-33 基金项目：国家支撑计划项目(2009BAI84B00) 作者简介：潘媛(1987-), 女, 博士研究生, 助理实验师, 研究方向:中药药效与毒理学, E-mail:panyuan1129@163.com *通讯作者：彭成, E-mail:pengchengchengdu@126.com 参考文献： 郭宇博. 2010. 不同配伍中药方剂对偏头痛大鼠血清和脑组织中5-HT含量的影响[J]. 中国中医基础医学杂