温度敏感型离子通道基因在哺乳动物内脏器官和血管中的表达研究
Expression Profiles of Genes Encoding Temperature-Sensitive Ion Channels in Mammalian Internal Organs and Blood Vessels

中文摘要：温度敏感型离子通道广泛存在于哺乳动物内脏器官和血管组织，在多种生理功能的调节中起重要作用。本研究以雄性比格犬和雄性SD大鼠的心脏、肝、脾、肾、肺、主动脉、肺动脉、肺静脉、股动脉、股静脉及颈总动脉为材料提取RNA，经逆转录PCR以及半定量方法获得这些通道基因的表达情况。结果显示：TRPV1在2种动物的上述组织中均有表达，而TRPV2和TRPM5在所有组织中均无表达；TRPV3仅表达在大鼠的肝、肺及肺动脉中，在狗的所有组织中均无表达；TRPV4在狗的肺中无表达，在2种动物的其他组织中均有表达；TRPM2在大鼠的主动脉、心脏、肺动脉中无表达，在2种动物的其他组织中均有表达；TRPM3在狗的肺、肺动脉及大鼠的肺、心脏、主动脉中无表达，在2种动物的其他组织中均有表达；TRPM4在狗的肺动脉以及大鼠的脾、肾、心脏中没有检测到表达，在2种动物的其他组织中均有表达；TRPM8在狗的肝、脾、肾以及大鼠的肾中无表达，在2种动物的其他组织中均有表达；TRPA1仅在大鼠的肺和脾中有表达。本研究比较全面地展示了温度敏感型离子通道基因在哺乳动物内脏器官和血管中的表达情况，为进一步探究其在哺乳动物生理调节中的功能奠定基础。
英文摘要：Temperature-sensitive ion channels widely exist in internal organs and blood vessels of mammals and play important roles in the regulation of various physiological functions. In this study, we collected tissues including the heart, liver, spleen, kidney, lung, aorta, pulmonary artery/vein, femoral artery/vein, and carotid artery from dogs and rats, respectively. Subsequently, the expression of genes encoding temperature-sensitive ion channels were detected using reverse transcription PCR and agarose gel electrophoresis. The results showed that TRPV1 existed in all the tissues of the two species examined, while TRPV2 and TRPM5 were undetectable in all the examined tissues. TRPV3 was expressed in the liver, lung and pulmonary artery of rat but absent in all canine tissues. TRPV4 existed in all the tissues of both species except in the lung of dog. TRPM2 was expressed in all the tissues of both species except in the aorta, heart and pulmonary artery of rat. TRPM3 expression was absent in the lung and pulmonary artery of dog and the lung, heart and aorta of rat, but presented in all other tissues. TRPM4 existed in all the tissues of both species except the pulmonary artery of dog and spleen, kidney and heart of rat. TRPM8 existed in all the tissues of both species except the liver, spleen and kidney of dog and kidney of rat. TRPA1 expressoin was found only in the lung and spleen of rat. These results provided detailed information for the expression of genes encoding temperature-sensitive ion channels in mammalian internal organs and blood vessels, which were essential for further exploration of physiological regulation by these channels. 2016,35(4): 517-522 收稿日期：2016-03-14 DOI：10.11984/j.issn.1000-7083.20160052 分类号：Q24;Q959.8 作者简介：刘浩然(1994-),男,本科生,E-mail:oromis@foxmail.com *通讯作者：曾博,E-mail:zengbo@swmu.edu.cn 参考文献： 蒲九君, 王志明, 周辉, 等. 2015. 瞬时感受器电位通道vanilloid 4在机械牵张人脑微血管内皮细胞中对内皮型一氧化氮合酶的作用[J]. 中南大学学报医学版, 40(9):960-966. Aijima R, Wang B, Takao T, et al. 2015. The thermosensitive TRPV3 channel contributes to rapid wound healing in oral epithelia[J].