高脂饮食联合链脲佐菌素对小鼠糖脂代谢及炎症的影响
Effect of High-fat Diet and Streptozotocin on the Glucose Lipid Metabolism and the Inflammatory in Mice

中文摘要：观察高脂饮食联合链脲佐菌素(STZ)对小鼠糖脂代谢及胰岛功能损伤的影响。将体质量为18~20 g的SPF级雄性C57BL/6J小鼠40只,随机分为对照组和模型组,每组20只。对照组小鼠饲喂对照饲料,模型组小鼠饲喂高脂饲料。1周后,禁食16 h,测量小鼠体质量与空腹血糖;尾静脉采血,分离血清;每周1次,连续4周。4周后,对照组小鼠腹腔注射柠檬酸缓冲液,模型组小鼠腹腔注射STZ,剂量为40 mg·kg -1,每天1次,连续3 d。继续饲养2周后,测量随机血糖,以小鼠血糖≥16.7 mmol·L -1者即判定为2型糖尿病。对照组及2型糖尿病小鼠经腹腔注射葡萄糖以进行糖耐量试验。与对照组同一时间的体质量和血糖进行比较,模型组小鼠体质量、血糖均升高,除第1周血糖外,差别均有统计学意义( P<0.05)。采用重复测量方差分析,发现喂养时间对体质量( F=200.831)和血糖( F=7.025)均有影响,差异有统计学意义( P<0.05);不同喂养时间对低密度脂蛋白( F=30.793)、甘油三酯( F=34.027)和高密度脂蛋白( F=30.793)均有影响,差异有统计学意义( P<0.05)。不同饲料喂养与不同喂养时间对体质量和甘油三酯存在交互作用( P<0.05)。比较糖耐量曲线发现,成模小鼠较对照组小鼠糖耐量降低。采用ELISA试剂盒检测小鼠血清中TNF-α含量,成模小鼠的TNF-α含量高于对照组,差异有统计学意义( P<0.05)。高脂饮食可导致C57BL/6J小鼠糖脂代谢紊乱,给予STZ后引起了小鼠糖耐量降低及炎症损伤,高脂饮食联合STZ可提高小鼠2型糖尿病模型的成模率。
英文摘要：To investigate the effect of high-fat diet combined with streptozotocin (STZ) on the injury glycolipid metabolism and inflammatory in mice. A total of 40 male C57BL/6J mice (18-20 g) were randomly divided into control group and model group (20 per group). The control group was fed as normal, and the model group was fed with high-fat diet. After one week, mice were fasted for 16 h, and the weight and fasting blood glucose were measured, the blood sample was collected from the tail vein for four weeks, once a week. The control group was injected with citric acid buffer and the model group was daily injected with STZ (40 mg·kg -1) for three days. After feeding with high fat diet for two weeks, random blood glucose was measured. The mice were defined as type 2 diabetes mellitus model if the blood glucose was more than 16.7 mmol·L -1. The model mice of both groups were treated with glucose by intraperitoneal injection for glucose tolerance test. Comparing with the control group, significant difference was observed in the weight and blood glucose of the model group after one week ( P<0.05). The effect of feeding time had significant effects on blood glucose ( F=7.025) and weight ( F=200.831) ( P<0.05) as determined by repeated measures variance analysis. The feeding time also had significant effects on low density lipoprotein ( F=30.793), triglyceride ( F=34.027) and the high density lipoprotein ( F=30.793) ( P<0.05). The feeding time and high-fat diet had interaction on the body weight and triglycerides ( P<0.05). The glucose tolerance of mice in model group decreased using the glucose tolerance curve. The serum TNF-α increased in model group ( P< 0.05). High-fat diet combined with STZ can lead to the disorder of glucose and lipid metabolism, reduce the glucose tolerance and inflammation in C57BL/6J mice. High-fat diet