家鸡G蛋白偶联受体78基因的克隆与组织表达图谱分析
Molecular Cloning and Tissue Expression of Chicken GPR78 Gene

中文摘要：G蛋白偶联受体(GPCR)是一大类膜受体超家族,参与了机体几乎所有的生理过程,是一类重要信号分子受体。GPR78作为GPCR超家族的成员之一,属于孤儿型受体。目前,在脊椎动物中,针对该基因结构与功能的研究极少。本研究以家鸡为动物模型,通过RT-PCR方法克隆了 GPR78基因的编码区序列,并探究了该基因在家鸡各组织中的表达情况。结果显示:家鸡 GPR78基因编码区全长为1020 bp,含3个外显子,可编码1个长为339个氨基酸的7次跨膜受体;该基因在家鸡脑各功能区及垂体中高表达,而在其他外周组织中均不表达。本研究为后续探究 GPR78基因在家鸡中的生理功能奠定基础。
英文摘要：G protein-coupled receptor (GPCR), also known as the seven transmembrane receptor, belongs to a large receptor gene superfamily and involves in nearly all physiological processes of organisms. GPR78, as a member of GPCR, is an orphan receptor. At present, little is known about the structure and biological functions of this receptor gene in vertebrates. In this study, using chicken as an animal model, we cloned the GPR78 gene and examined its expression in different tissues of chicken. The results showed that the open reading frame of chicken GPR78 was 1020 bp in length and contained three exons, and this gene was predicted to encode a receptor of 339 amino acids; RT-PCR assay revealed that chicken GPR78 was highly expressed in various brain regions and pituitary, but not in other examined peripheral tissues. The data of study will contribute to explore the physiological roles of GPR78 gene in chickens. 2016,(1): 52-57 收稿日期：2015-05-12 DOI：10.11984/j.issn.1000-7083.20150178 分类号：Q78 基金项目：国家自然科学基金项目(31271325; 31272436) 作者简介：胡媛媛(1989-), 女, 硕士研究生, 研究方向为动物繁殖与分子内分泌, E-mail:1043945258@qq.com *通讯作者：李娟, E-mail:lijuanhk@gmail.com 参考文献： 何夏萍, 周彦妮, 阎振鑫, 等. 2013. 猪黑皮质素受体3和4基因克隆及分析[J]. 四川大学学报 (自然科学版), 50(4): 899-907. 徐雪, 张剑南, 张耀荣, 等. 2014. 猪GPR84基因克隆和组织表达图谱分析[J]. 四川大学学报 (自然科学版), 51(5): 1079-1084. 张彦, 于晓婕, 李娟, 等. 2014. 家鸡卵清蛋白基因调控序列克隆与分析[J]. 四川大学学报 (自然科学版), 51(3): 609-614. Altamura AC, Boin F, Maes M. 1999. HPA axis and cytokines dysregulation in schizophrenia: potential implications for the antipsychotic treatment[J]. European Neuropsychopharmacology, 10(1): 1-4. Brown AJ, Goldsworthy SM, Barnes AA, et al. 2003. The Orphan G protein-coupled receptors GPR41 and GPR43 are activated by propionate and other short chain carboxylic acids[J]. The Journal of Biological Chemistry, 278 (13): 11312-11319. Fredriksson R, Schioth HB. 2005. The repertoire of G-protein-coupled receptors in fully sequenced genomes[J]. Molecular Pharmacology, 67(5): 1414-1425. Herman JP, Prewitt CM, Cullinan WE. 1996. Neuronal circuit regulation of the hypothalamo-pituitary-adrenocortical stress axis[J]. Critical Reviews in Neurobiology, 10: 371-394. Jones PG, Nawoschik SP, Sreekumar K, et al. 2007. Tissue distribution and functional analyses of the constitutively active orphan G protein coupled