美洲大蠊成虫肠道菌群多样性分析
Analysis on the Intestinal Microbiota Diversity of Adult Periplaneta americana

中文摘要：美洲大蠊 Periplaneta americana既是卫生害虫，也是重要的药用昆虫。许多研究表明，其肠道菌群种类和数量与宿主健康密切相关。本实验利用16S rRNA高通量测序技术分析了人工饲养的美洲大蠊成虫肠道菌群。结果得到有效序列67 492条，归类为581个操作分类单元，注释到21门79目193属。厚壁菌门Firmicutes （56.96%）和拟杆菌门Bacteroidetes （33.13%）为美洲大蠊成虫肠道的优势菌门。葡萄球菌属 Staphylococcus（16.59%）、芽孢杆菌属 Bacillus（13.26%）和副杆菌属 Parabacteroides（10.26%）为美洲大蠊成虫肠道的优势菌属。研究结果为进一步探讨美洲大蠊肠道菌群多样性以及与宿主之间的关系打下了基础。
英文摘要： Periplaneta americana is not only an important pest affecting human health, but also a medicinal economic insect. Many studies have suggested that the type and quantity of intestinal microbiota are closely related to the host health. In this study, 16S rRNA high-throughput sequencing technique was used to analyze the intestinal microbiota of artificially fed P. americana. The results showed that a total of 67 492 16S rRNA sequences classified as 581 operational taxonomic units, annotated to 193 genera, 79 orders and 21 phyla, were obtained. Specifically, among the identified species in the intestinal microbiota of P. americana, Firmicutes (56.96%) and Bacteroidetes (33.13%) were the dominant phyla, Bacteroidales (32.11%), Bacillales (29.89%), Clostridiales (12.93%) and Lactobacillales (10.73%) were the dominant orders, Staphylococcus (16.59%), Bacillus (13.26%) and Parabacteroides (10.26%) were the dominant genera, and Staphylococcus sciuri (14.95%) was the most prevalent species. These results laid a solid foundation for further exploring the intestinal microbiota diversity and its relationship with the host P. americana. 2018,37(4): 415-419 收稿日期：2018-03-12 DOI：10.11984/j.issn.1000-7083.20180081 分类号：Q969.25 基金项目：四川好医生攀西药业有限责任公司资助项目 作者简介：郝艳芹(1989-),女,硕士研究生,研究方向:分子生态学,E-mail:1849853004@qq.com *通讯作者:岳碧松,E-mail:bsyue@scu.edu.cn 参考文献： 段荟芹, 王利. 2015. 耗儿鱼松鼠葡萄球菌的分离鉴定及耐药性分析[J]. 中国畜牧兽医, 42(5):1288-1293. 李钧敏, 金则新. 2006. 一种高效可直接用于PCR分析的土壤总微生物DNA抽提方法[J]. 应用生态学报, 17(11):2107-2111. 刘晓飞, 刘娟, 田蕾, 等. 2015. 美洲大蠊成虫肠道可培养细菌多样性研究[J]. 微生物学通报, 42(11):2207-2214. 张晓杰, 莫晓畅, 莫建初. 2016. 白蚁共生放线菌研究进展[J]. 环境昆虫学报, 38(2):437-444. 张振宇, 圣平, 黄胜威, 等. 2017. 昆虫肠道菌群的多样性、功能及应用[J]. 氨基酸和生物资源, 39(4):231-239. Bagde US, Uma G, Ram P. 2013. Isolation and characterization of gut-associated microbes in cockroach[J]. African Journal of Microbiology Research, 7(19):2034-2039. Bertino-Grimaldi D, Medeiros MN, Vieira RP, et al. 2013. Bacterial community composition shifts in the gut of Periplaneta americana, fed on different lignocellulosic materials[J]. SpringerPlus, 2:609. DOI:https://doi.org/10.1186/2193-1801-2-609. Carrasco P, Pérez-Cobas AE, van de PC. 2014. Succession of the gut microbiota in the cockroach Blattella germanica[J].