牦牛肠源 Lactobacillus acidophilusL3对其肠道分泌型免疫球蛋白A及免疫相关因子的影响
Effect of Intestinal Lactobacillus acidophilus L3 on the SIgA and Immune Factors of Small Intestine in Yaks

中文摘要：为了研究牦牛肠源 Lactobacillus acidophilus L3(嗜酸乳杆菌)对其肠道分泌型免疫球蛋白A(SIgA)及免疫相关因子的影响,将10头健康牦牛(2~2.5岁)随机分为2组,分别为益生菌组和空白对照组,益生菌组在饲料中添加2×10 9 CFU·kg -1 L. acidophilus L3,饲喂28 d后取其小肠样品,ELISA检测试验组和对照组十二指肠、空肠、回肠中SIgA、白细胞介素-2(IL-2)、IL-4、IL-6、干扰素γ(IFN-γ)的含量.研究结果表明, L. acidophilus L3可有效增加试验组牦牛肠道SIgA的分泌量( P <0.05),组内比较发现SIgA在回肠含量最高,其次为空肠和十二指肠.IL-2、IL-4、IL-6在试验组小肠中的表达量较对照组显著增加( P <0.05),试验组小肠IFN-γ的表达量较对照组降低( P <0.05).证实 L. acidophilus L3可提高牦牛肠道的黏膜免疫功能.
英文摘要：In order to study the effects of Lactobacillus acidophilus L3 on the intestinal SIgA and immune factors of yaks, 10 yaks aged from 2 to 2.5 years were used in this study, and 5 of which were fed daily with L. acidophilus L3 (a concentration of 2×10 9 CFU·kg -1 feed), while the others were conventional feed without adding L. acidophilus L3. Small intestinal samples were collected on day 28, and the content of SIgA, IL-2, IL-4, IL-6, IFN-γ in duodenum, jejunum, and ileum were detected by ELISA. The results showed that the content of intestinal SIgA was significantly increased in the probiotic group compared with the control ( P <0.05). The highest content of SIgA was found in ileum followed by jejunum and duodenum. The content of intestinal IL-2, IL-4 and IL-6 were also significantly increased in the probiotic group compared with the control ( P <0.05). The content of intestinal IFN-γ was decreased in the probiotic group compared with the control ( P <0.05). These results indicated that L. acidophilus L3 can effectively improve the intestinal mucosal immune function of yaks by increasing the levels of intestinal SIgA, IL-2, IL-4 and IL-6. 2015,34(3): 411-414 收稿日期：2014-11-5 DOI：10.3969/j.issn.1000-7083.2015.03.016 分类号：Q959.8;S85 基金项目：陕西省教育厅科学研究计划(自然科学专项)项目2013JK0721;陕西理工学院人才启动院项目SLGQD13-19;陕西理工学院人才启动院项目SLGQD14-01;农业部兽用药物创制重点实验室和甘肃省新兽药工程重点实验室开放课题1610322011011 作者简介：祁珊珊(1984—),女,博士,讲师,主要从事动物病理学研究,E-mail：qishanshan101@126.com *通讯作者：郑红星,博士,讲师,主要从事药理毒理学研究,E-mail：zhenghongxing100@126.com 参考文献： 曹晋宜, 韩军丽, 王友湘, 等. 2009. 瑞士乳杆菌对小鼠肠道黏膜和肠组织中细胞因子影响的研究[J]. 食品科学, 30(21): 343-347. 高源, 张振, 王宝香. 2011. 嗜酸乳杆菌对感染人轮状病毒乳鼠肠黏膜的保护作用[J]. 世界华人消化杂志, 19(28): 2963-2969. 刘冬, 刘沛. 2004. 肠道分泌型IgA的成分及功能[J]. 世界华人消化杂志, 12: 411-415. 祁珊珊, 郑红星. 2014. 动物肠道黏膜免疫系统研究进展[J]. 黑龙江畜牧兽医, 7: 2963-2969. 朱于敏, 潘道东, 邹思湘, 等. 2005. 灌喂不同储存期酸奶对主动免疫小鼠免疫功能的影响[J]. 食品科学, 26(4): 229-232. Blaise C. 2013. Role of secretory IgA in infection and maintenance of homeostasis[J]. Autoimmunity Reviews, 12(6): 661-665. Perdigón G, Alvarez S, Oliver G, et al. 1986. Effect of perorally administered Lactobacilli on macrophage activation in mice[J]. Infection and Immunity,