钙结合蛋白在龟类听觉核团的表达
Expression of Calcium-binding Proteins on the Auditory Nuclei in Turtles

中文摘要：在漫长的演化过程中,物种对环境的适应和选择,形成了多样化的听觉系统结构.本研究以钙结合蛋白为标记,利用免疫组织化学的方法,对龟鳖类的底栖和浅水代表物种(中华鳖 Pelodiscus sinensis和红耳龟 Trachemys scripta elegans)的中枢神经系统的听觉核团进行了对比研究.研究结果不但展示了2个物种的听觉核团的横切面结构,并且表明了2个物种的层状核团存在关键的结构差异,而这样的差异可能与其各自生境的声音传播特点密切相关.
英文摘要：During the long evolution process the auditory nerve system of animals has been diversified into different structures according to natural selection. The immunohistochemistry of calcium-binding proteins was selected in this study on the auditory nuclei between demersal and adlittoral turtles ( Pelodiscus sinensis and Trachemys scripta elegans). The results showed cross sections of auditory nuclei of the two species. And the structural difference of nucleus laminaris was proved by different media of sound. 2015,34(3): 321-329 收稿日期：2014-4-1 DOI：10.3969/j.issn.1000-7083.2015.03.001 分类号：Q959.6 基金项目：中国科学院"百人计划"项目(KSCX2-YW-R-077);青年自然科学基金项目(31301882);中国博士后基金项目(2013M542301) *通讯作者：唐业忠,E-mail：tangyz@cib.ac.cn 参考文献： Belekhova M, Chudinova T, Kenigfest N, et al. 2008. Distribution of metabolic activity (cytochrome oxidase) and immunoreactivity to calcium-binding proteins in the turtle brainstem auditory nuclei[J]. Journal of Evolutionary Biochemistry and Physiology, 44: 354-364. Belekhova M, Chudinova T, Repéran J, et al. 2010. Core-and-belt organisation of the mesencephalic and forebrain auditory centres in turtles: Expression of calcium-binding proteins and metabolic activity[J]. Brain Research, 1345: 84-102. Belekhova M, Kenigfest N, Minakova M, et al. 2003. Calcium-binding proteins in the turtle thalamus. analysis in the light of hypothesis of the "core-matrix" thalamic organization in relation to the problem of homology of thalamic nuclei among amniotes[J]. Journal of Evolutionary Biochemistry and Physiology, 39(6): 624-647. Belekhova M, Kenigfest N, Karamian O, et al. 2004. Distribution of calcium-binding proteins in the central and peripheral regions of the turtle mesencephalic center torus semicircularis[J]. Doklady Biological Sciences, 399: 451-454. Boord R, Rasmussen G. 1963. Projection of the cochlear and lagenar nerves on the cochlear nuclei of the pigeon[J]. Journal of Comparative Neurology, 120(3): 463-475. Boord R. 1968. Ascending projections of the primary cochlear nuclei and nucleus laminaris in the pigeon[J]. Journal of Comparative Neurology, 133(4): 523-541. Braun K. 1990. Calcium-binding proteins in avian and mammalian central nervous system: localization, development and possible functions[J]. Progress in Histochemistry and Cytochemistry, 21: 1-64. Braun K, Scheich H, Hezmann C, et al. 1991. Parvalbumin