康定湍蛙物种界定
Species Delimitation of Amolops kangtingensis

中文摘要：康定湍蛙 Amolops kangtingensis依据多地混合标本订立,其分类地位一直存在争议。本文从居群水平出发,涵盖其多个模式产地及分布区,联合染色体核型、形态数量性状、分子系统发育分析,对其有效性及物种进行界定。结果表明,以新都桥为模式产地的康定湍蛙成立,分布区域大致界定于折多山以西雅砻江流域,海拔3000 m及以上高原地区,而原康定湍蛙大渡河流域各居群应划归四川湍蛙 A. mantzorum物种之中。
英文摘要： Amolops kangtingensis was described on the samples from different areas, and the validity of this species was controversial. On population level including several type localities and their distribution areas, combined use of karyotype analysis, morphological measurements and molecular phylogeny was conducted to delimit this species in this study. The results suggested that A. kangtingensis was a valid species with type locality in Hsintuchiao. And its distribution areas were delimitated in the district along Yalong River at altitude above 3000 m. However, the populations distributed along Tatu River should be merged into A. mantzorum. 2015,34(6): 801-809 收稿日期：2015-04-02 DOI：10.11984/j.issn.1000-7083.20150122 分类号：Q959.5 作者简介： *通讯作者：曾晓茂,E-mail:zengxm@cib.ac.cn 参考文献： 蔡红霞, 赵尔宓. 2008. 中国横断山区湍蛙属4个物种有效性的探讨[J]. 四川动物, 27(4): 483-488. 蔡红霞. 2008. 湍蛙亚科Amolopsinae (sensu Yang, 1991)的系统发育学研究[D]. 成都: 四川大学. 费梁, 胡淑琴, 叶娼媛, 等. 2009. 中国动物志: 两栖纲(下卷)(无尾目 蛙科)[M]. 北京: 科学出版社: 1538-1545. 费梁, 叶昌媛, 黄永昭. 2005. 中国两栖动物检索及图解[M]. 四川: 四川科学技术出版社: 150-158. 刘承钊, 胡淑琴. 1961. 中国无尾两栖类[M]. 北京: 科学出版社: 226-243. 吴贯夫, 谭安鸣, 赵尔宓. 1987. 康定湍蛙为一有效种的细胞学证据[J]. 两栖爬行动物学报, 6(4): 9-41. 吴贯夫, 赵尔宓. 1984. 无尾类两种罕见的核型——四川湍蛙及凉山湍蛙的核型[J]. 两栖爬行动物学报, 3(4): 5-10. 吴政安, 谭安鸣, 赵尔宓. 1987. 横断山区四种湍蛙的细胞遗传学研究[J]. 遗传学报, 14(1): 63-68. Barley AJ, White J, Diesmos AC, et al. 2013. The challenge of species delimitation at the extremes: diversification without morphlogical logical change in Philippine Sun Skink[J]. Evolution, 67(12): 3556-3572. Dubois A. 1992. Notes sur la classification des Ranidae (Amphibiens; Anoures)[J]. Bull mens Soc Linn Lyon, 61: 305-352. Frost DR. 2014. Amphibian species of the world: an online reference. version 5.6[EB/OL].(2013-01-09). http://research.amnh.org/herpetology/amphibia/index.html. Hall TA. 1999. Bioedit: a user-friengly biological sequence alignment editor an analysis program for Windows 95/98NT[J]. Nycleicacids Symposium Series, 41: 95-98. Huelsenbeck JP, Ronquist F. 2001. MrBayes: Bayesian inference of phylogeny[J]. Bioinformatics, 17: 754-755. Levan A, Fredga K, Sandberg A. 1964. Nomenclature for centromeric position on chromosomes[J]. Hereditas, 5(2): 201-220. Librado P, Rozas J. 2009. DnaSP v5: a software for comprehensive analysis of DNA polymorphism data[J]. Bioinformatics, 25(11): 1451-1452. Liu CC. 1950. Amphibian of western China[M]. Fieldiana Zool Mem Chicago, 2: